FIGURE 8

JC-1 vital staining of rat cardiomyocytes exposed to oxidative stress. Non-pretreated and pepstatin-A-pretreated (100 m) cells were exposed to 1.5 m naphthazarin for various amounts of time and then stained with 5 g/ml JC-1 for 10 minutes. Mitochondria are green (_{m m} < -100 mV) and orange-red (_{m m} > -140 mV) in control cells but only green in cells with a decreased _m. Cells containing green or red/green mitochondria were counted, and the illustrated values are means sd, n = 4 (500 cells/sample). Cells pretreated with pepstatin A and then subjected to naphthazarin differed significantly from cells exposed solely to naphthazarin, as calculated by the Mann-Whitney U test. ^* p 0.05.