Laboratory Investigation

FIGURE 6

FROM:

Localization of Tissue Transglutaminase in Human Carotid and Coronary Artery Atherosclerosis: Implications for Plaque Stability and Progression

Zishan A Haroon, Thomas Wannenburg, Madhu Gupta, Charles S Greenberg, Reidar Wallin and David C Sane

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Figure 6.

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TG activity standard curve. A colorimetric assay for transglutaminase was established, using guinea pig liver transglutaminase (GPTG) as the standard enzyme. The assay is based on the incorporation of 5-biotinamindopentylamine into immobilized N, N'-dimethylcasein, as described in "Materials and Methods." The assay was linear between 0 and 1 mug/ml of GPTG. This assay was used to determine the activity of transglutaminase in coronary artery atherectomy tissue (Table 2).

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