FIGURE 3
FROM:
Bombesin Receptors in Distinct Tissue Compartments of Human Pancreatic Diseases
Achim Fleischmann, Ursula Läderach, Helmut Friess, Markus W Buechler and Jean Claude Reubi
BACK TO ARTICLEFigure 3.
High affinity and specificity of the 125I-[Tyr4]-bombesin binding in a displacement experiment. Sections containing GRP receptor-positive parenchyma in chronic pancreatitis samples or peritumoral vessels in pancreatic adenocarcinoma samples were incubated with 125I-[Tyr4]-bombesin and increasing concentrations of unlabeled bombesin (
), GRP (
), neuromedin B (
), and somatostatin-14 (
). Optical density was measured in parenchyma and vessels respectively, using a computer-assisted image processing system. High affinity displacement of the tracer is found with bombesin and GRP whereas neuromedin B shows lower affinity. Somatostatin 14 has no effect. Nonspecific binding was subtracted from all values. The observed rank order of potencies of analogs corresponds to the GRP receptor.
