George F Murphy

Welcome to Laboratory Investigation

Advancing the understanding of human and experimental disease

Latest Research

  • Article |

    We found that ZYX was correlated with glioma progression and worse prognosis of patients and identified ZYX as a biomarker for diagnosis. Our study provided insights on ZYX functions and revealed that ZYX played an important role in the invasion of GBM probably through regulating the expression of STMN1.

    • Xian-Mei Wen
    • , Tao Luo
    • , Yi Jiang
    • , Li-Hong Wang
    • , Ying Luo
    • , Qian Chen
    • , Kaidi Yang
    • , Ye Yuan
    • , Chunhua Luo
    • , Xiang Zhang
    • , Ze-Xuan Yan
    • , Wen-Juan Fu
    • , Yu-Huan Tan
    • , Qin Niu
    • , Jing-Fang Xiao
    • , Lu Chen
    • , Jiao Wang
    • , Jia-Feng Huang
    • , You-Hong Cui
    • , Xia Zhang
    • , Yan Wang
    •  & Xiu-Wu Bian
  • Article |

    Epithelial–mesenchymal transition (EMT) plays a critical role in the progression of salivary gland (SG) fibrosis in primary Sjögren’s syndrome. This study demonstrates the IL-17-dependent activation of EMT in human SG epithelial cells that occurs through both the canonical TGF-β1/Smad2/3 and noncanonical TGF-β1/Erk1/2 pathways.

    • Margherita Sisto
    • , Loredana Lorusso
    • , Giuseppe Ingravallo
    • , Domenico Ribatti
    •  & Sabrina Lisi
  • Article |

    Dermatitis is a chronic inflammatory skin disorder. Nax is a sodium channel that regulates inflammatory gene expression when the barrier function of the skin is disrupted. Knockdown of Nax relieves dermatitis symptoms in rabbit dermatitic skin. Nax is a novel therapeutic target for dermatitis, which currently has limited treatment options.

    • Jingling Zhao
    • , Shengxian Jia
    • , Ping Xie
    • , Emily Friedrich
    • , Robert D. Galiano
    • , Shaohai Qi
    • , Renxiang Mao
    • , Thomas A. Mustoe
    •  & Seok Jong Hong
  • Article |

    Aberrant proliferation is a central hallmark of polycystic kidney diseases (PKDs) but the role of specific G1-phase cyclin-dependent kinases (Cdks) is not clear. This study shows that the kinetics of Cdk2 and Cdk2-cyclin partners do not correlate with proliferation and that the absence of Cdk2 does not alter renal cyst growth, most likely due to compensation by Cdk1. These findings therefore suggest that Cdk2 is not required for the progression of PKD.

    • Jennifer Qin Jing Zhang
    • , Jane Burgess
    • , Daria Stepanova
    • , Sayanthooran Saravanabavan
    • , Annette T. Y. Wong
    • , Philipp Kaldis
    •  & Gopala K. Rangan
  • Article |

    Calcitonin gene-related peptide (CGRP) regulates inflammation through receptor activity-modifying protein 1 (RAMP1), a subunit of CGRP receptor. This study reveals that RAMP1 signaling in immune cells enhances lipopolysaccharide-induced lymphangiogenesis in the diaphragm, which contributes to improving lymphatic drainage from peritoneal fluid.

    • Seri Tsuru
    • , Yoshiya Ito
    • , Hiromi Matsuda
    • , Kanako Hosono
    • , Tomoyoshi Inoue
    • , Shuji Nakamoto
    • , Chie Kurashige
    • , Toshiaki Mishima
    • , Kazutake Tsujikawa
    • , Hirotsugu Okamoto
    •  & Masataka Majima
  • Article
    | Open Access

    Mass Spectrometry Overcomes PD-L1 IHC Limitations Associated with Aged Tissue. PD-L1 IHC was evaluated in stored tissue with mass spectrometry (MS). Increased humidity and temperature resulted in considerable immunoreactivity loss, particularly among antibodies recognizing extracellular and discontinuous epitopes, which could be partially mitigated with the use of desiccant. However, even in significantly affected tissues MS was able to quantitate PD-L1 expression and assess peptide oxidation. These results suggest MS is suited for biomarker assessments using stored sections—a sample type commonly encountered in oncology research.

    • Alexander Haragan
    • , Daniel C. Liebler
    • , Dimple M. Das
    • , Michael D. Soper
    • , Ryan D. Morrison
    • , Robbert J. C. Slebos
    • , Bradley L. Ackermann
    • , Jeff A. Fill
    • , Andrew E. Schade
    • , John R. Gosney
    •  & Aaron M. Gruver

News & Comment

Supplement

United States & Canadian Academy of Pathology 108th Annual Meeting Abstracts: Unlocking Your Ingenuity

The largest gathering of physician-pathologists in the world