Laboratory Investigation

Kidney International (1975) 7, 154–160; doi:10.1038/ki.1975.23

Effect of diphosphonate on crystallization of calcium oxalate in vitro

Charles Y C Pak1, Masahiro Ohata1 and Karen Holt1

1Department of Internal Medicine, Southwestern Medical School, The University of Texas Health Science Center at Dallas, Dallas, Texas

Correspondence: Dr Charles Y C Pak, Department of Internal Medicine, Southwestern Medical School, the University of Texas Health Science Center at Dallas, Dallas, Texas 75235, U.S.A.

Received 1 June 1974; Revised 16 September 1974.

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Abstract

The effect of diphosphonate on crystallization of calcium oxalate in vitro. Reliable techniques for the calculation of activity product (state of saturation), formation product (limit of metastability) and crystal growth of calcium oxalate were devised. The activity product at saturation was 2.53 times 10–-9 M2, and was independent of duration of incubation, solid-to-solution ratio, pH, calcium concentration or ionic strength. These techniques were utilized to assess the effect of disodium ethane-1-hydroxy-1, 1-diphosphonate (EHDP) on crystallization of calcium oxalate in an aqueous salt solution in vitro. EHDP increased the formation product of calcium oxalate, indicating an inhibition of nucleation of calcium oxalate. Further, it inhibited the crystal growth of calcium oxalate.

Effet du diphosphonate sur la cristallisation de l'oxalate de calcium in vitro. Des techniques fiables ont été mises au point pour le calcul du produit de solubilité(état de saturation), du produit de formation (limite de métastabilité) et de la croissance cristalline de l'oxalate de calcium. Le produit de solubilité à saturation est 2,53 times 10 –-9 M2, il est indépendant de la durée de l'incubation, du rapport solide/solution, du pH, de la concentration du calcium et de la force ionique. Ces techniques ont été utilisées pour évaluer les effets du disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) sur la cristallisation de l'oxalate de calcium en solution aqueuse in vitro. EHDP augmente le produit de formation de l'oxalate de calcium ce qui indique une inhibition de la nucléation de l'oxalate de calcium. De surcroît il inhibe la croissance cristalline de l'oxalate de calcium.

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References

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