FIGURE 3

(A) Complete amino acid content (after hydrolysis) of mass fractions isolated from normal urine by size-exclusion chromatography. Results are means from the analysis of urine specimens from 5 individuals. Amino acids are expressed as mol/mmol creatinine. (B) As for (A), but omitting the fraction of <250 Da, which contains primarily free amino acids, and with 80-fold increase in scale. Abbreviations for amino acids are: G, glycine; D+N, L-aspartic acid and L-asparagine; T, L-threonine; S, L-serine; E+Q, L-glutamic acid and L-glutamine; A, L-alanine; V, L-valine; M, L-methionine; I, L-isoleucine; L, L-leucine; Y, L-tyrosine; F, L-phenylalanine; H, L-histidine; K, L-lysine; R, L-arginine; HO-K, hydroxylysine; MeHis, 1- and 3-methyl-L-histidines; HO-P, hydroxy-L-proline; P, L-proline.