FIGURE 1

Acute diabetes suppresses protein degradation in renal cortical suspensions. Sprague-Dawley rats were given a single intravenous injection of 125 mg/kg streptozotocin (DM) and then pair-fed with vehicle-injected control (Con) rats for 3 (A) or 7 days (B). The rate of protein degradation was measured as lysine release on cortical suspensions (see Methods section). Results for individual pair-fed animals are indicated by a line. P < 0.05 for both time points, N = 6 at 3 days, N = 5 at 7 days. (C) Six DM rats and their pair-fed controls were treated for 7 days and had their kidneys pooled for cortical suspensions. The suspensions were divided and 10 mmol/L NH₄Cl was added during the protein degradation measurement to one-half the sample. The results shown are those of a representative experiment of two repeats.