Clinical Investigation

Kidney International (1974) 6, 184–190; doi:10.1038/ki.1974.97

Radioimmunoassay of urinary antidiuretic hormone in man: Stimulation–suppression tests

Philippe Fressinaud, Pierre Corvol, Joël Menard and with the technical assistance of Jacqueline Allegrini

Centre Hopitale Universitaire d'Angers, Angers, and INSERM, Unite 36, Paris, France

Correspondence: Dr Jöel Ménard, 17 rue du Fer à Moulin, 75005 Paris, France.

Received 11 March 1974; Revised 29 May 1974.

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Abstract

Radioimmunoassay of urinary antidiuretic hormone in man: Stimulation-suppression tests. Urinary vasopression (AVP) has been measured in human beings by a sensitive and reproducible radioimmunoassay. An orally administered water load (15 ml/kg) in dehydrated normal subjects increases CH2O from -1.4 plusminus 0.1 to 2.5 plusminus 0.6 ml/min while urinary AVP decreases from 7.82 plusminus 1.56 to 2.69 plusminus 0.40 ng/2 hr. Infusion of hypertonic saline decreases CH2O from -1.8 plusminus 0.3 to -2.2 plusminus 0.3 ml/min and increases urinary AVP from 8.24 plusminus 2.75 to 20.28 plusminus 1.94 ng/2 hr. The correlation between all the observed values for CH2O and urinary AVP is highly significant (r = -0.66, N = 26, P < 0.001). The correlation between plasma osmolality and urinary AVP is also significant (r = 0.62, N = 26, P < 0.001). According to this equation, AVP begins to appear in urine for a plasma osmolality of 275.2 mOsm/kg. During infusion of hypertonic saline, plasma renin (PRA) and plasma aldosterone (PA) have been measured by radioimmunoassays. While hypertonic saline infusion stimulates vasopressin release, it decreases PRA and PA. AVP excretion as measured by radioimmunoassay of urinary AVP responds to alterations in plasma osmolality which affect AVP release, and is an effective and precise means of assessing neurophyseal function in man. Variations in renin secretion and AVP release are dissociated during hypertonic saline load.

Dosage radioimmunologique de l'hormone antidiurétique urinaire chez l'homme: Tests de stimulation-freination. L'arginine vasopressine urinaire (AVP) a été dosée chez l'homme par une méthode radioimmunologique sensible et reproductible. Une charge en eau (15 ml/kg) chez des sujets déshydratés augmente CH2O de -1,4 plusminus 0,1 à 2.5 plusminus 0,6 ml/min et diminue l'AVP urinaire de 7,82 plusminus 1,56 à 2,69 plusminus 0,40 ng/2 hr. La perfusion de sérum salé hypertonique diminue CH2O de -1,8 plusminus 0,3 à -2,2 plusminus 0,3 ml/min et augmente l'AVP urinaire de 8,24 plusminus 2,75 à 20,28 plusminus 1,94 ng/2 hr. Il existe une corrélation hautement significative entre les valeurs de CH2O et celles d'AVP urinaire (r = -0.66, N = 26, P < 0.001). La corrélation entre l'osmolalité plasmatique et l'AVP urinaire est très significative (r = 0.62, N = 26, P < 0.001) et selon cette équation l'AVP commence à apparaître dans les urines pour une osmolalité plasmatique de 275.2 mOsm/kg. Pendant la perfusion de sérum salé hypertonique, la rénine plasmatique (ARP) et l'adostérone plasmatique (AP) ont été mesurées par méthodes radioimmunologiques. Alors que le sérum salé stimule la sécrétion de vasopressine, il diminue l'ARP et l'AP. L'excretion d'AVP mesurée par dosage radioimmunologique de l'AVP urinaire répond aux modifications de l'osmolalité plasmatique qui affectent la sécrétion d'AVP, et reflète donc avec précision la fonction neurohypophysaire. Les variations de sécrétion de la rénine et de l'AVP sont dissociées pendant la charge en sel.

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