FIGURE 5

Frequency distribution of the area fraction parameter. A plot of the data clearly separates the group exposed to the high glucose concentration for four hours from the other experimental mice (***P < 0.001). Failure of data to pass the Kolmogorov-Smirnov normality test (P < 0.001) indicates that the frequency distribution of all groups significantly varied from the pattern given by the numbers obtained from a normally distributed population of data. This manifestation reflects the expected and characteristic heterogeneity of a cell population reacting, in vivo and in situ, to the experimental intervention. In other words, at variance with the synchronized standpoint peculiar of the in vitro setup, a cell population in vivo covers a whole range of stages within each individual cell life cycle. Symbols are: () HBSS after 2 to 3 and 4 hours; () 4.25% glucose after 2 hours; () 4.25% glucose after 3 hours; () 4.25% glucose after 4 hours.