FIGURE 6
FROM:
Kidney cortex cells derived from SV40 transgenic mice retain intrinsic properties of polarized proximal tubule cells
Cécile Chalumeau, Danièle Lamblin, Soline Bourgeois, Pascale Borensztein, Régine Chambrey, Patrick Bruneval, Jean Paul Duong Van Huyen, Marc Froissart, Juerg Biber, Michel Paillard, Odile Kellermann and Josiane Poggioli
BACK TO ARTICLEFigure 6.
cAMP response to parathyroid hormone (PTH) and arginine vasopressin (AVP) in mouse kidney cortical cells (MKCCs). (A) Hormone-induced cAMP accumulation. cAMP was measured in confluent cells in the absence or presence of PTH and AVP. Cells were preincubated for 15 minutes with 1 mM IBMX, and then PTH or AVP (10-7
M) were added for an additional 15 minutes in the continual presence of IBMX. Values are means 
SE of seven experiments. *P < 0.001. (B) Sensitivity of the Na-phosphate uptake to 10-7
M PTH. Values are means SE of three experiments. *P < 0.05. (C) Sensitivity of NHE-3 activity to PTH 10-7
M. PTH was present during each step of the acidifying protocol.22Na uptake was performed in the presence of 10 mM Na. It was linear over five minutes. Na/H activity was assayed by outward proton gradient-stimulated initial rate of 22Na uptake after cell acidification by NH4 prepulse, as described in the Methods section. Results are means SE of five experiments. *P < 0.02 by paired t-test.
