Kidney International

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Aluminum is a weak agonist for the calcium-sensing receptor

Robert F Spurney, Min Pi, Patrick Flannery and L Darryl Quarles

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Figure 1.

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Western blot analysis of calcium sensing receptor (CaSR) expression in human embryonic kidney (HEK) 293 cells. Expression of rat CaSR was assessed by Western blotting, as described in the Methods section, in human parathyroid tissue (lane 1), in HEK 293 cells stably transfected with the CaSR cDNA (lane 2), and in nontransfected HEK 293 cells (lane 3). We detected three major bands of apparent molecular mass of 140, 165, and 250 kDa in membrane protein preparations from HEK 293 transfected with CaSR cDNA. These bands were not observed in nontransfected HEK 293 cells. The 250 and 165 kDa bands predominated in human parathyroid tissue.

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