FIGURE 6
FROM:
Goodpasture antigen: Expression of the full-length 
3(IV) chain of collagen IV and localization of epitopes exclusively to the noncollagenous domain
ANU LEINONEN, KAI-OLAF NETZER, ARIEL BOUTAUD, SRIPAD GUNWAR and BILLY G HUDSON
BACK TO ARTICLEFigure 6.
The assessment of binding capacity of Goodpasture (GP) antibodies to the r-
3(IV) chain and the r-3(IV)NC1 domain by binding of GP antibodies by r-3(IV)NC1 affinity column. The r-
3(IV) chain (A and C) and r-3(IV)NC1 (B and D) were coated on the plate. GP antibodies (GP-1), which bound to the r-3(IV)NC1 affinity column (A and B) and unbound antibodies (C and D) were preincubated with various concentrations of r-3(IV) chain, r-3(IV)NC1, r-2(IV)NC1, and albumin before adding them to the coated wells. Color development was measured at 410 nM.
