Kidney International

FIGURE 5

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High glucose alters the response of mesangial cell protein kinase C isoforms to endothelin-1

EMILY ANNE GLOGOWSKI, EVANGELIA TSIANI, XIAOPENG ZHOU, IVAN GEORGE FANTUS and CATHARINE WHITESIDE

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Figure 5.

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PKC-delta immunofluorescence. Mesangial cells growth-arrested on glass cover slips were cultured in normal (5.6 mM) glucose or high (30 mM) glucose for 48 hours, followed by 10 minutes of stimulation with endothelin-1 (ET-1; 100 nM). PKC-delta was detected in fixed, permeabilized cells by confocal scanning immunofluorescence microscopy using monoclonal anti-PKC–delta antibody and FITC-conjugated secondary antibody (magnification times240).

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