FIGURE 4
FROM:
High glucose alters the response of mesangial cell protein kinase C isoforms to endothelin-1
EMILY ANNE GLOGOWSKI, EVANGELIA TSIANI, XIAOPENG ZHOU, IVAN GEORGE FANTUS and CATHARINE WHITESIDE
BACK TO ARTICLEFigure 4.
(Upper panel) Representative paired immunoblots of mesangial cell PKC-
in the cytosol and particulate fractions following 48 hours in normal glucose (NG; 5.6 mM) or high glucose (HG; 30 mM) and 10-minute stimulation with phorbol 13-myristate 12-acetate (PMA; 100 nM) or endothelin-1 (ET-1; 100 nM). Rat brain was used as a positive control. (Lower panel) PKC-
immunoblot densitometry data (mean 
SE, N = 5 experiments) from mesangial cell cytosol (
) and particulate (
) fractions presented as percentage above NG basal (100%). *P < 0.05 vs. NG basal; **P < 0.05 vs. NG + ET-1.
