Cell Biology - Immunology - Pathology

Kidney International (1997) 52, 387–392; doi:10.1038/ki.1997.345

Mesangial cell abnormalities in spontaneously hypertensive rats before the onset of hypertension

José B Lopes de Faria1, Driss Zoukhri1 and Mara Lorenzi1

1Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, USA

Correspondence: Mara Lorenzi MD, Schepens Eye Research Institute, Boston, Massachusetts, 02114, USA. E-mail: lorenzi@vision.eri.harvard.edu

Received 22 August 1996; Revised 27 February 1997; Accepted 3 March 1997.

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Abstract

Mesangial cell abnormalities in spontaneously hypertensive rats before the onset of hypertension. To identify kidney biosynthetic abnormalities that may precede the onset of hypertension, we studied the expression of flbronectin (FN) and collagen IV (Coll IV) in young SHR (4 weeks of age) whose systolic blood pressure was normal and similar to that of age-matched control WKY rats. In isolated glomeruli the level of FN protein assessed by immunoblotting tended to be lower in the SHR than in the WKY rats. By Northern analysis the FN/actin mRNA ratio was significantly lower in glomeruli from SHR (0.56 plusminus 0.47) than in glomeruli from WKY rats (2.0 plusminus 0.8). These abnormalities were maintained in vitro since the expression of FN was significantly lower in SHR than in WKY cultured mesangial cells (FN/actin mRNA ratio = 0.84 plusminus 0.46 vs. 1.9 plusminus 0.7, P = 0.029). No differences in Coll IV mRNA or protein levels were observed in SHR glomeruli and mesangial cells when compared with WKY rats. The levels of aortic FN and Coll IV mRNAs were not different in SHR and WKY rats. In addition, mesangial cells from SHR showed a significantly higher growth rate than those from WKY. The biosynthetic and proliferative abnormalities observed in the SHR mesangial cells appear to reflect genetic characteristics, and could provide novel insights into cellular mechanisms linking the genetics of hypertension with predisposition to glomerular pathology.

Keywords:

hypertension, mesangial cell, genetics and hypertension, diabetes

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