Clinical Investigation

Kidney International (1996) 50, 1337–1343; doi:10.1038/ki.1996.446

IL-1bold beta, a major stimulator of hyaluronan synthesis in vitro of human peritoneal mesothelial cells: Relevance to peritonitis in CAPD

Susan Yung1, Gerald A Coles1 and Malcolm Davies1

1Institute of Nephrology, University of Wales College of Medicine, Royal Infirmary, Cardiff, Wales, United Kingdom

Correspondence: Dr Malcolm Davies, Institute of Nephrology, University of Wales College of Medicine, Royal Infirmary, Cardiff CF2 1SZ, Wales, United Kingdom.

Received 22 February 1996; Revised 13 May 1996; Accepted 13 May 1996.

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Abstract

IL-1beta, a major stimulator of hyaluronan synthesis in vitro of human peritoneal mesothelial cells: Relevance to peritonitis in CAPD. The effect of several different growth factors and cytokines on the synthesis of hyaluronan (HA) by human peritoneal mesothelial cells (HPMC) was investigated. Growth arrested HPMC synthesized low levels of HA, but co-culture with PDGF-bb, TGF-beta1, TNF-alpha, and IL-6 at a concentration of 10 ng/ml all increased HA synthesis between two- to three-fold. At the same concentration IL-1beta significantly increased the synthesis eight-fold (N = 3; P < 0.05). The effect of IL-1beta was also dose- and time-dependent and could be totally negated with interleukin-1 receptor antagonist (IL-1beta RcA). Non-infected and infected dialysate from patients receiving CAPD was also found to stimulate HA synthesis by HPMC. The levels found with non-infected fluid were 4 times 104 dpm/ml (N = 6) and 12.9 times 104 dpm/ml (N = 6; P < 0.002) and 8.7 times 104 dpm/ml (N = 6; P < 0.003) for infected fluid collected one and two days after the commencement of peritonitis. IL-1beta RcA dramatically reduced the effect of infected but not non-infected dialysate. These results provide new insights into the manner in which HA synthesis is controlled in the mesothelium and suggest that IL-1beta is a key cytokine in the inflammatory response in CAPD patients.

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