Laboratory Investigation

Kidney International (1974) 5, 261–270; doi:10.1038/ki.1974.35

Proximal tubular lactate transport in rat kidney: A micropuncture study

Bernhard Höhmann, Peter P Frohnert, Rolf Kinne, Karl Baumann, with the technical assistance of F Papavassiliou and M Wagner

Max Planck Institut für Biophysik, Frankfurt/Main, Germany

Correspondence: Dr K Baumann, Max Planck Institut für Biophysik, D6 Frankfurt/Main 70, Kennedy-Allee 70, Germany.

Received 4 June 1973; Revised 22 September 1973.

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Abstract

Proximal tubular lactate transport in rat kidney: A micropuncture study. Micropuncture studies of renal lactate behavior in the rat showed free filtration across the glomerular membrane. Under free-flow conditions, 95% of the filtered load was reabsorbed by the proximal tubule, thus generating a transtubular concentration gradient. In the absence of volume changes, an intratubular steady-state concentration of lactate was established irrespective of the presence or absence of lactate in the initial test solution, indicating a passive "leak" along the transtubular concentration gradient which increased with higher serum lactate levels. Although proximal tubular hydrogen ion secretion could explain this lactate gradient on the basis of nonionic diffusion, studies in chronic alkalotic rats showed that lactate was reabsorbed even in the absence of a hydrogen ion gradient. Inhibition of intracellular gluconeogenesis from lactate, by alkalosis and through administration of an inhibitor (MICA), abolished development of the transtubular concentration gradient in the proximal tubule. It is concluded that lactate reabsorption in the proximal tubule is caused by simple diffusion across the luminal membrane in response to a concentration gradient created by intracellular utilization of lactate for gluconeogenesis.

Transport tubulaire proximal du lactate par le rein de rat: Etude par microponctions. L'étude par microponction du comportement rénal du lactate chez le rat a montré une filtration libre à travers la membrane glomérulaire. Dans des conditions de flux libre, 95% de la charge filtrée est réabsorbée par le tube proximal contre un gradient de concentration trans-tubulaire. En l'absence de modifications de volume un état stationnaire de concentration du lactate est établi indépendamment de la présence ou de l'absence de lactate dans la solution initiale, ce qui indique un fuite passive du lactate le long du gradient trans-tubulaire de concentration, fuite qui augmente en même temps que la concentration plasmatique du lactate. Une secrétion tubulaire proximale d'ions hydrogène pourrait expliquer ce gradient de lactate en fonction d'une diffusion non ionique. Cependant l'étude de rats en alcalose métabolique a montré que le lactate est réabsorbé même en l'absence d'un gradient d'ions hydrogène. L'inhibition de la gluconéogénèse intracellulaire à partir du lactate par Palcalose et au moyen de l'administration d'un inhibiteur (MICA) abolit ce gradient trans-tubulaire proximal. Il est conclu que la réabsorption du lactate dans le tube proximal est liée à une diffusion simple à travers la membrane luminale en fonction d'un gradient de concentration créé par l'utilisation intracellulaire de lactate pour la gluconéogénèse.

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