Laboratory Investigation

Kidney International (1994) 45, 692–699; doi:10.1038/ki.1994.93

Effects of neurohypophyseal antagonists in postnephrectomy natriuresis in male rats

Wan Huang1, Siu-Lan Lee1 and Mats Sjöquist1

1Department of Physiology and Medical Biophysics, Biomedicum, Uppsala University, Uppsala, Sweden

Correspondence: Mats Sjöquist, Departments of Physiology and Medical Biophysics, Biomedicum, Uppsala University, Box 572, S-751 23 Uppsala, Sweden.

Received 13 October 1992; Revised 20 September 1993; Accepted 20 September 1993.

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Abstract

Effects of neurohypophyseal antagonists in postnephrectomy natriuresis in male rats. Acute unilateral nephrectomy (AUN) in anesthetized male Lewis x DA rats induced rapid and consistent increases in electrolyte and fluid excretion by the remaining kidney during the first hours. Continuous infusion of a vasopressin (AVP) V1-receptor antagonist d(CH2)5 Tyr(Me)AVP (V1-ant) reduced renal electrolyte and fluid excretion before and after AUN to a similar extent, whereas an oxytocin (OT)-receptor antagonist [Mpa1,D-Tyr(Et)2,Thr4,Orn8]-OT (CAP) at the same dose selectively attenuated the increase in sodium excretion after AUN. The plasma concentration of OT rose significantly after AUN (9.16 plusminus 1.4 to 21.45 plusminus 5.07 pg dot ml-1). A similar OT level obtained by infusion of OT mimicked the renal responses to AUN without elevating blood pressure; however, only CAP but not V1-ant efficiently reversed OT-induced natriuresis. Also, the infusion of CAP at the same dose produced no effects on the rise of blood pressure caused by AVP while the infusion of the V1-ant prevented such a rise. Thus, CAP reduced the natriuresis after AUN by interfering with OT-and not V1-receptors. In conclusion, evidence is presented, for the first time, concerning the major role of OT receptors in the acute readjustment of the renal sodium excretion after AUN, and a synergistic role for AVP in terms of the general magnitude of renal excretion.

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