Laboratory Investigation

Kidney International (1994) 45, 788–793; doi:10.1038/ki.1994.104

Measurement of intracellular generation of hydrogen peroxide by rat glomeruli in vitro

Norishi Ueda1, Bertrand Guidet1 and Sudhir V Shah1

1Department of Internal Medicine, Division of Nephrology, University of Arkansas for Medical Sciences and John L. McClellan Memorial Veterans Administration Hospital, Little Rock, Arkansas, USA

Correspondence: Sudhir V Shah MD, University of Arkansas for Medical Sciences, 4301 W. Markham, Slot 501, Little Rock, Arkansas 72205, USA.

Received 23 July 1993; Revised 15 October 1993; Accepted 25 October 1993.

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Abstract

Sequential reduction of oxygen along the univalent pathway leads to the generation of superoxide anion, hydrogen peroxide, hydroxyl radical, and water [1, 2]. These partially reduced oxygen intermediates have been implicated as important mediators in various models of ischemic, toxic and immune-mediated tissue injury including glomerular injury [3]. Reactive oxygen metabolites have been shown to affect several biological processes potentially important in glomerular diseases, and their role in both inflammatory as well as non-inflammatory glomerular diseases has recently been demonstrated [3]. Evidence for the importance of reactive oxygen metabolites in experimental models of glomerular disease is based largely on the protective effects of scavengers of reactive oxygen metabolites.

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