Clinical Investigation

Kidney International (1991) 40, 285–290; doi:10.1038/ki.1991.212

Stimulation of beta2-microglobulin synthesis in lymphocytes after exposure to Cuprophan dialyzer membranes

Bernhard Jahn, Margarita Betz, Reinhold Deppisch, O Janssen, Gerhard Maria Hänsch and Eberhard Ritz

Institute of Immunology, and Department of Internal Medicine, Ruperto Carola University, Heidelberg, Germany

Correspondence: E Ritz MD, Department of Internal Medicine, Ruperto Carola University, Bergheimerstrasse 58, D-6900 Heidelberg, Germany.

Received 21 March 1990; Revised 11 March 1991; Accepted 19 March 1991.

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Abstract

Stimulation of beta2-microglobulin synthesis in lymphocytes after exposure to Cuprophan dialyzer membranes. We tested beta2-microglob-ulin (beta2m) synthesis by peripheral blood lymphocytes of non-uremic donors after contact with dialyzer membranes. Under serum-free conditions, lymphocytes were incubated with different dialyzer membranes for four hours. After subsequent culture in the absence of membranes to yield an overall culture time of 24 hours, RNA was extracted. Messenger RNA for beta2m was quantitated by Northern blotting with specific probes. Increased beta2m mRNA was seen after a minimum of one hour contact time with Cuprophan both in the presence and absence of cycloheximide. While no consistent stimulation was seen with polyacrylonitrile or polycarbonate-polyether membranes, respectively, the stimulation of beta2m mRNA with Cuprophan was of the same order of magnitude as that obtained with interferon gamma. Stimulation of beta2m mRNA by Cuprophan was stereospecifically diminished by 5 mM L-fucose. Apart from stimulated transcription of beta2m we could also show increased surface expression of MHC class I molecules, using F ACS technique. The results further prove complement-independent blood cell activation by Cuprophan membranes. It is uncertain whether the results are relevant for beta2m amyloidosis.

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