Clinical Investigation

Kidney International (1973) 4, 401–406; doi:10.1038/ki.1973.136

The effect of diphosphonate on calcium phosphate crystallization in urine in vitro

Masahiro Ohata1 and Charles Y C Pak1

1Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, Texas

Correspondence: Dr Charles Y C Pak, Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, Texas 75235, U.S.A.

Received 5 May 1973; Revised 15 August 1973.

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Abstract

The effect of diphosphonate on calcium phosphate crystallization in urine in vitro. Disodium ethane-1-hydroxy-1, 1-diphosphonate (EHDP) was added to human urine in vitro and tested for its effect on nucleation, crystal growth and crystal dissolution of brushite (CaHPO4 dot 2H2O), the probable nidus for certain calcium-containing renal stones. The EHDP (0.5 to 8 mg of P/liter) increased the formation product of brushite, indicating an inhibition of nucleation of brushite. It inhibited the crystal growth of brushite in every urine specimen tested. Finally, it facilitated the crystal dissolution of brushite in some urine specimens. Thus, EHDP might be clinically useful in the prevention of renal stone formation.

Effet du diphosphonate sur la cristallisation du phosphate de calcium dans l'urine in vitro. Du disodium éthane-1-hydroxy-1, 1-diphosphonate (EHDP) a été ajouté in vitro à de l'urine humaine et l'on a étudié ses effets sur la nucléation, la croissance cristalline et la dissolution cristalline de la brushite (CaHPO4 dot 2H2O), le germe probable de certains calculs urinaires contenant du calcium. L'EHDP (0,5–0,8 mg P/litre) augmente le produit de formation de la brushite, ce qui indique une inhibition de sa nucléation. Il inhibe la croissance cristalline de la brushite dans toutes les urines étudiées. Enfin, il facilité la dissolution cristalline de la brushite dans quelques échantillons d'urine. Ainsi, l'EHDP peut peut-être avoir une utilité clinique dans la prévention des calculs urinaires.

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References

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