Clinical Investigation

Kidney International (1990) 37, 951–954; doi:10.1038/ki.1990.70

Growth hormone and growth hormone binding protein in human urine

Naoki Hattori, Akira Shimatsu, Yuzuru Kato and Hiroo Imura

Second Division, Department of Internal Medicine, Kyoto University Faculty of Medicine, Kyoto 606, and the First Division, Department of Internal Medicine, Shimane Medical University, Izumo 693, Japan

Correspondence: Naoki Hattori MD, Second Division, Department of Internal Medicine, Kyoto University Faculty of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606, Japan.

Received 26 June 1989; Revised 10 October 1989; Accepted 18 October 1989.

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Abstract

Growth hormone and growth hormone binding protein in human urine. The presence of growth hormone binding protein (GH-BP) in urine was demonstrated in normal subjects. Scatchard analysis showed that an association constant (Ka) and a maximum binding capacity (Bmax) of urinary GH-BP were 2.3 plusminus 0.8 x 108 M-1 and 19.2 plusminus 4.0 ng/mg urinary protein, respectively. The molecular weight of the urinary GH-BP was estimated to be 50000 to 70000 on gel chromatography. Although the molecular weight and Ka were similar to those of plasma GH-BP, Bmax of urinary GH-BP was significantly higher than that of plasma GH-BP (0.44 plusminus 0.12 ng/mg protein). GH-BP in urine may be derived from plasma and/or renal tubules.

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