Laboratory Investigation

Kidney International (1990) 37, 875–879; doi:10.1038/ki.1990.61

A study of growth regulators of renal cortical tubular cells in the rabbit liver

Shigeru Kanda1, Koichiro Nomata1, Pabitra K Saha1, Naoki Nishimura1, Jun Yamada1, Hiroshi Kanetake1 and Yutaka Saito1

1Department of Urology, Nagasaki University School of Medicine, Nagasaki 852, Japan

Correspondence: Shigeru Kanda MD, Department of Urology, Nagasaki University School of Medicine, Nagasaki 852, Japan.

Received 3 July 1989; Revised 10 October 1989; Accepted 18 October 1989.

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Abstract

A study of growth regulators of renal cortical tubular cells in the rabbit liver. Two growth regulators, a growth stimulator for cultured renal tubular cells and a growth inhibitor, were observed in this study in rabbit liver homogenate after a unilateral nephrectomy. These regulators appeared in the liver on the third day after this nephrectomy, decreased on the seventh day, and disappeared by the fourteenth day. The growth stimulatory activity, termed a tubular cell growth factor (TuCGF), was a heat- and acid stable 15 to 20 KDa protein. The additive effects of TuCGF on the epidermal growth factor (EGF), the insulin-like growth factor-I (IGF-I), and the fibroblast growth factor (FGF) were observed; the results suggest that TuCGF differs from EGF, IGF-I, or FGF. In contrast, the growth inhibitory activity, termed a tubular cell growth inhibitor (TuCGI), was a heat- and acid-labile protein with a molecular weight of about 150 to 200 KDa. This factor potently inhibited the DNA synthesis of tubular cells in the presence of insulin and EGF. These results suggest that TuCGI is not identical with transforming growth factor-beta (TGF-beta). The regulation of these activities by target cell density is also discussed.

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