Laboratory Investigation

Kidney International (1990) 37, 727–735; doi:10.1038/ki.1990.39

Transport of organic compounds in renal plasma membrane vesicles of cadmium intoxicated rats

Hye Young Lee1, Kyoung Ryong Kim1, Jae Suk Woo1, Yong Keun Kim1 and Yang Saeng Park1

1Departments of Physiology, Kosin Medical College, and Pusan National University College of Medicine, Pusan, Korea

Correspondence: Dr Yang Saeng Park, Department of Physiology, Kosin Medical College, 34 Amnam-dong, Suh-ku, Pusan 602-030, Korea.

Received 1 February 1989; Revised 28 July 1989; Accepted 15 September 1989.

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Abstract

Transport of organic compounds in renal plasma membrane vesicles of cadmium intoxicated rats. Effects of cadmium intoxication on renal transport systems for various organic compounds were studied. Subcutaneous injections of CdCl2 (2 mg Cd/kg dot day) for two to three weeks induced marked polyuria, glycosuria, and proteinuria without altering glomerular filtration rate. In renal cortical brush border membrane vesicles (BBMV) isolated from cadmium treated rats, Na+-dependent D-glucose uptake was markedly attenuated, and this was due to reduction in Vmax and not Km. Likewise, Na+-driven L-glutamate transport and H+-driven tetraethylammonium transport were significantly reduced. In renal cortical basolateral membrane vesicles (BLMV) of cadmium intoxicated rats, Na+-dependent succinate transport was drastically reduced. These results indicate that cadmium intoxication impairs various transport systems for organic compounds in the brush border and basolateral membranes of proximal renal tubules.

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