Clinical Investigation

Kidney International (1989) 36, 712–718; doi:10.1038/ki.1989.251

Prostaglandin-mediated suppression of in vitro growth of erythroid progenitor cells

Shuichi Taniguchi1, Tsunefumi Shibuya1, Mine Harada1 and Yoshiyuki Niho1

1The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan

Correspondence: Tsunefumi Shibuya MD, The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Maidashi 3-1-1 Higashi-ku, Fukuoka 812, Japan.

Received 2 September 1988; Revised 21 March 1989; Accepted 8 May 1989.

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Abstract

Prostaglandin-mediated suppression of in vitro growth of erythroid progenitor cells. In vitro hematopoiesis was evaluated in 37 patients with chronic renal failure (CRF) who developed moderate to severe anemia in order to clarify the relationship between the growth of erythroid progenitor cells and CRF-associated anemia. Bone marrow cells from these patients were cultured in the presence of recombinant erythropoietin. Both early and late erythroid progenitor cells (BFU-E and CFU-E) were significantly suppressed in patients with CRF compared to those in normal controls, while myeloid progenitor cells (GM-CFC) remained normal. Suppression of CFU-E was shown to be mediated by prostaglandin(s) secreted from bone marrow adherent cells. Furthermore, the suppression of CFU-E was inversely correlated with concentrations of uremic serum or parathyroid hormone added to the assay system. These observations suggest a possibility that late erythroid progenitor cells may be preferentially suppressed by the network consisting of parathyroid hormone, bone marrow adherent cells and prostaglandin(s).

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References

  1. Eschbach JW, Adamson JW: Anemia of end-stage renal disease (ESRD). Kidney Int 28:1–5, 1985 | PubMed | ISI | ChemPort |
  2. Radtke HW, Clausler A, Erbes PM, Scheurmann EH, Schoeppe W, Koch KM: Serum erythropoietin concentration in chronic renal failure: Relationship to degree of anemia and excretory renal function. Blood 54:877–884, 1979 | PubMed | ISI | ChemPort |
  3. Caro J, Brown S, Miller O, Murray T, Erslev AJ: Erythropoietin levels in uremic nephric and anephric patients. J Lab Clin Med 93:449–458, 1979 | PubMed | ISI | ChemPort |
  4. Jacobs K, Shoemarker C, Rudersdorf R, Neill S, Kaufman RJ, Mufson A, Seehra T, Jones S, Hewick R, Fritsch EF, Kawakita M, Shimizu T, Miyake T: Isolation and characterization of genomic and cDNA clones of human erythropoietin. Nature 313:806–810, 1985 | Article | PubMed | ISI | ChemPort |
  5. Winearls CG, Olivwer OO, Pippard MJ, Reid C, Downing MR, Cotes PM: Effect of human erythropoietin derived from recombinant DNA on the anaemia of patients maintained by chronic haemodialysis. Lancet ii:1175–1177, 1986
  6. Eschbach JW, Egrie JC, Downing MR, Browne JK, Adamson JW: Correction of the anemia of end-stage renal disease with recombinant human erythropoietin. Results of a combined phase I and II clinical trial. N Engl J Med 316:73–78, 1987 | PubMed | ISI | ChemPort |
  7. Shaw AB: Haemolysis in chronic renal failure. Br Med J 2:213–216, 1967
  8. Radtke HW, Rege AB, LaMarche MB, Bartos D, Bartos F, Campbell RA, Fisher JW: Identification of spermine as an inhibitor of erythropoiesis in patients with chronic renal failure. J Clin Invest 67:1623–1629, 1981 | PubMed |
  9. Freedman MH, Cattran DC, Saunders EF: Anemia of chronic renal failure: Inhibition of erythropoiesis by uremic serum. Nephron 35:15–19, 1983
  10. Strickland ID, Chaput de Saintonge DM, Boulton FE, Francis B, Roubikova J, Waters JI: The therapeutic equivalence of oral and intravenous iron in renal dialysis patients. Clin Nephrol 7:55–57, 1977 | PubMed | ISI | ChemPort |
  11. Hampers CL, Steiff R, Nathan DG, Snyder D, Merill JP: Megaloblastic hematopoiesis in uremia and in patients on long-term hemodialysis. N Engl J Med 276:551–554, 1967
  12. Wills MR, Savory J: Alminum poisoning: Dialysis encephalopathy, osteomalacia, and anemia. Lancet i:29–34, 1983
  13. Massry SG: Is parathyroid hormone a uremic toxin? Nephron 19:125–130, 1977
  14. Massry SG, Goldstein DA: The search for uremic toxin(s) "X" : "X" x PTH. Clin Nephrol 11:181–189, 1979
  15. Meytes D, Bogin E, Ma A, Dukes P, Massry SG: Effect of parathyroid hormone on erythropoiesis. J Clin Invest 67:1263–1269, 1981 | PubMed | ISI | ChemPort |
  16. Avram MM, Alexix H, Rahman M, Son B, Iancu M: Decreased transfusional requirement following parathyroidectomy in long term hemodialysis. (abstract) Proc Am Soc Nephrol 5:5, 1971
  17. Mladenovic E, Eschbach JW, Garcia JF, Adamson JW: The anaemia of chronic renal failure in sheep: Studies in vitro. Br J Haematol 58:491–500, 1984
  18. Delwiche F, Segal GM, Eschbach JW, Adamson JW: Hematopoietic inhibitors in chronic renal failure: Lack of in vitro specificity. Kidney Int 29:641–648, 1986 | PubMed | ChemPort |
  19. Warren MK, Ralph P: Macrophage growth factor CSF-1 stimulates human monocyte production of interferon, tumor necrosis factor, and myeloid CSF. J Immunol 137:2281–2285, 1986 | PubMed |
  20. Horiguchi J, Warren MK, Donald K: Expression of the macrophage-specific colony-stimulating factor in human monocytes treated with granulocyte-macrophage colony stimulating factor. Blood 69:1259–1261, 1987
  21. Sullivan R, Gans PJ, McCarroll LA: The synthesis and secretion of granulocyte-monocyte colony-stimulating activity (CSA) by isolated human monocytes: Kinetics of the response to bacterial endotoxin. J Immunol 130:800–807, 1983 | PubMed | ChemPort |
  22. Rambaldi A, Young DC, Griffin JD: Expression of the M-CSF (CSF-1) gene by human monocytes. Blood 69:1409–1413, 1987 | PubMed | ISI | ChemPort |
  23. Kurland JI, Bockman RS, Broxmeyer HE, Moore MAS: Limitation of excessive myelopoiesis by the intrinsic modulation of macrophage-derived prostaglandin E. Science 199:552–555, 1978 | PubMed | ISI | ChemPort |
  24. Kurland JI, Broxmeyer HE, Pelus LM, Brockman RS, Moore MAS: Role for monocyte-macrophage-derived colony-stimulating factor and prostaglandin E in the positive and negative feedback control of myeloid stem cell proliferation. Blood 52:388–407, 1978 | PubMed | ChemPort |
  25. Shibuya T, Mak TW: Host control of susceptibility to erythroleukemia and to the types of leukemia induced by Friend murine leukemia virus: initial and late stages. Cell 31:483–493, 1982 | PubMed |
  26. Masunaga H, Goto M, Ueda M: Effect of recombinant erythropoietin on in vivo hemopoiesis. Acta Haematol Jpn 50:1119–1125, 1987
  27. Taniguchi S, Shibuya T, Morioka E, Okamura T, Okamura S, Inaba S, Niho Y: Demonstration of three distinct immunological disorders on erythropoiesis in a patient with pure red cell aplasia and autoimmune haemolytic anaemia associated with thymoma. Br J Haemat 68:473–477, 1988
  28. Messner HA, Till TE, McCulloch EA: Interacting cell population affecting granulopoietic colony formation by normal and leukemic human bone marrow cells. Blood 42:701–710, 1973
  29. Myers RF, Siegel MI: Differential effects of anti-inflammatory drugs on lipoxygenase and cyclooxygenase activities of neutro phils. Biochem Biophys Res Commun 112:586–594, 1983
  30. Konagaya Y, Konagaya M, Takatsuki K, Tomita A, Watanabe S, Noda T, Katsuragi S: Studies of circulating parathyroid hormone in man by a homologous amino-terminal specific radioimmunoassay. Jap J Med 23:199–204, 1984
  31. Ohno Y, Rege AB, Fisher JW, Barona J: Inhibitors of erythroid colony-forming cells (CFU-E and BFU-E) in sera of azotemic patients with anemia of renal disease. J Lab Clin Med 92:916–923, 1978
  32. Urabe A, Chiba S, Kosaka K, Takaku F: Response of uraemic bone marrow cells to erythropoietin in vitro. Scand J Haematol 7:335–340, 1976
  33. Snyder DS, Desforges JF: Lipoxygenase metabolites of arachidonic acid modulate hemopoiesis. Blood 67:1675–1679, 1986 | PubMed | ChemPort |
  34. Ross GB, Migliaccio AR, Migliaccio G, Lettieri F, Dirosa M, Mastroberardins G, Peschle C: In vitro interactions of PGE and cAMP with murine and human erythroid precursors. Blood 56:74–79, 1980
  35. Chan HSL, Saunders EF, Freedman MH: Modulation of human hematopoiesis in prostaglandins and lithium. J Lab Clin Med 95:125–132, 1980
  36. Fisher JW, Hagiwara M: Effect of prostaglandins on erythropoiesis. Blood Cell 10:241–260, 1984
  37. Neal WA, Garver FA, Lewis JP, Gardner E, Luthcher CL: Partial characterization of an erythropoiesis inhibitory factor. Biochem Med 33:8–16, 1985

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