Laboratory Investigation

Kidney International (1988) 34, 631–637; doi:10.1038/ki.1988.227

Angiotensin influences on tubuloglomerular feedback mechanism in hypertensive rats

Wann-Chu Huang, P Darwin Bell, David Harvey, Kenneth D Mitchell and L Gabriel Navar

Nephrology Research and Training Center, and Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama; and Department of Physiology and Biophysics, National Defense Medical Center, Taipei, Taiwan, Republic of China

Correspondence: L Gabriel Navar PhD, Renal Physiology Lab, 727 Sparks Center, University of Alabama at Birmingham, University Station, Birmingham, Alabama 35294, USA.

Received 14 August 1986; Revised 22 June 1988.

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Abstract

Angiotensin influences on tubuloglomerular feedback mechanism in hypertensive rats. The tubuloglomerular feedback (TGF) mechanism was evaluated in the nonclipped kidney of Goldblatt hypertensive rats from both stop flow pressure (SFP) and single nephron glomerular filtration rate (SNGFR) responses to step increases in late proximal perfusion rate from 0 to 40 nl/min. During control conditions, increases in late proximal perfusion rate produced flow dependent decreases in SFP and SNGFR with maximal values of 10.2 plusminus 1.0 mm Hg and 12.9 plusminus 2.5 nl/min, values similar to those obtained in normal rats. During ACE inhibition (MK 422; 0.6 mg/kg/hr), arterial pressure decreased from 168 plusminus 8 to 137 plusminus 7 mm Hg and there was a marked attenuation in the magnitude of SFP feedback responses (Delta = 2.5 plusminus 0.3 mm Hg). SNGFR feedback responses, however, were not significantly impaired. Direct decreases in renal arterial pressure reduced control SFP but SFP feedback responses were maintained, indicating that the attenuated SFP feedback responses during ACE inhibition were not due to decreased arterial pressure. Superimposed infusion of angiotensin II during ACE inhibition partially restored SFP feedback responses. In contrast, norepinephrine infusion did not result in a similar restoration of SFP feedback sensitivity. These results indicate that the nonclipped kidney of Goldblatt hypertensive rats has an intact TGF mechanism as assessed from SFP and SNGFR feedback responses. Furthermore, ACE inhibition attenuates SFP but not SNGFR feedback responses, and systemic angiotensin II infusions can restore SFP feedback responsiveness towards normal.

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