Clinical Investigation

Kidney International (1988) 34, 109–116; doi:10.1038/ki.1988.152

Identification of glomerular immune deposits in cryoglobulinemia glomerulonephritis

Renato A Sinico, Christopher G Winearls, Ettore Sabadini, Alessandro Fornasieri, Aldo Castiglione and Giuseppe D'Amico

Division of Nephrology, Ospedale San Carlo Borromeo, Milano, Italy, and Department of Medicine, Hammersmith Hospital, London, United Kingdom

Correspondence: Dr R A Sinico, Division of Nephrology, San Carlo Hospital, Via Pio II, 3, 20153 Milano, Italy.

Received 23 October 1987; Revised 11 February 1988.

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Abstract

Identification of glomerular immune deposits in cryoglobulinemia glomerulonephritis. To provide further evidence of the nature of intraglomerular immune deposits in essential mixed cryoglobulinemia (EMC), we used two mouse monoclonal antibodies against cross-reactive idiotypes present on monoclonal rheumatoid factors (MoRFs) from patients with type II-EMC. MoAb Cc1 reacted with 9 of 16 circulating IgMk MoRFs tested, and MOAb Lc1 with four of the remaining. Using indirect immunofluorescence and immunoperoxidase techniques, we could identify the same cross-reactive idiotype of the serum MoRF in the renal biopsy specimens from 11 of 13 patients with EMC glomerulonephritis. Kidney specimens from the three patients, whose MoRF was not recognized by MoAbs Cc1 and Lc1, were negative. Two out of 30 control renal biopsies from patients with other forms of glomerulonephritis were shown to contain idiotype (Cc1 and Lc1) positive material. Both patients had serum polyclonal RF which could account for this finding. In conclusion, our results provide direct evidence that serum cryo-MoRF participate in the formation of glomerular immune deposits and, presumably, in the pathogenesis of renal damage in EMC glomerulonephritis.

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