Laboratory Investigation

Kidney International (1988) 34, 26–35; doi:10.1038/ki.1988.141

Interleukin 1 and the glomerular mesangium. III. IL-1-dependent stimulation of mesangial cell protein kinase activity

David H Lovett, Michael Martin, Stuart Bursten, Marta Szamel, Diethard Gemsa and Klaus Resch

Institut für Molekularpharmakologie, Medizinische Hochschule Hannover, Hannover, Federal Republic of Germany; Medical Service, Seattle VAMC-University of Washington, Seattle, Washington, USA; and Institut für Immunologie, Universität Marburg, Marburg, Federal Republic of Germany

Correspondence: D H Lovett MD, Medical Service 111J, San Francisco Veterans Administrations Medical Center, 4150 Clement Street, San Francisco, California 94121, USA.

Received 7 May 1987; Revised 11 December 1987.

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Abstract

Interleukin 1 and the glomerular mesangium. III. IL-1-dependent stimulation of mesangial cell protein kinase activity. Interleukin 1 (IL-1) exerts a number of biologic actions upon cultured glomerular mesangial cells (MC). These include stimulation of cellular proliferation and induction of prostaglandin and type IV collagenase secretion. It was determined that this activity, as with other polypeptide growth factors, was associated with the activation of specific MC plasma membrane protein kinases. Plasma membranes from cycling MC were incubated with purified IL-1 and (32P) ATP in the absence of calcium and cyclic nucleotides. Macrophage IL-1 stimulated the rapid phosphorylation of several plasma membrane proteins, the most significant of which were 52–55 kd, 46 kd, and 20 kd in size. Macrophage IL-1 induced specific membrane phosphorylation in concentrations as low as 1.5 times 10-12 M, an effect obtained with equivalent concentrations of purified MC IL-1. The 46 kd phosphoprotein, which was the most prominent, was alkali-resistant and contained phosphotyrosine when examined by phosphoamino acid analysis. The 52–55 kd and 20 kd phosphoproteins were alkali-labile and contained phosphoserine. The 46 kd phosphoprotein was the major phosphoprotein recovered from Con A-Sepharose and IL-1 affinity columns. Induction of plasma membrane-associated protein kinase activity may represent one mechanism whereby IL-1 initiates mesangial cellular activation.

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