Laboratory Investigation

Kidney International (1988) 34, 13–17; doi:10.1038/ki.1988.139

Effects of aldosterone on NEM-sensitive ATPase in rabbit nephron segments

Lal C Garg1 and Neelam Narang1

1Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida, USA

Correspondence: Dr L C Garg, Department of Pharmacology, Box J-267 JHM Health Center, Gainesvile, Florida 32610, USA.

Received 1 July 1987; Revised 8 December 1987.

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Abstract

Effects of aldosterone on NEM-sensitive ATPase in rabbit nephron segments. Aldosterone (aldo) treatment of animals stimulates the rate of H+ secretion in the collecting duct, a process which may involve an H+-ATPase sensitive to inhibition by NEM (N-ethylmaleimide). Therefore, we determined NEM-sensitive ATPase activity in distal nephron segments from three groups of adrenaiectomized (adx) rabbits maintained on different doses of aldo (in an osmotic minipump) for seven days. Group 1 was given 1.5 microg aldo/100 g body wt/day, whereas groups 2 and 3 were maintained on 5 microg and 50 microg of aldo/100 g body wt/day, respectively. Aldo concentrations in the plasma of groups 1, 2 and 3 were 10.4 plusminus 0.8, 70 plusminus 7 and 408 plusminus 133 ng/dl, respectively. There was a significant increase in NEM-sensitive ATPase activity in connecting tubule (CNT) and cortical, outer and inner medullary duct segments (CCD, OMCD and IMCD) but not in cortical thick ascending limb (CTAL) and distal convoluted tubule (DCT) in group 2 as compared to group 1. A further increase in plasma concentration of aldo (group 3) did not produce any more increase in NEM-sensitive ATPase activity in the CNT, CCD, OMCD and IMCD, but did increase the enzyme activity in the DCT. These results are consistent with the hypothesis that aldo increases H+ secretion in the connecting tubule and collecting duct segments by increasing the activity of NEM-sensitive H+-ATPase activity in these segments.

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