Technical Note

Kidney International (1988) 33, 897–899; doi:10.1038/ki.1988.82

A method for isolation of mouse glomeruli for quantitation of immune deposits

V Joyce Gauthier1 and Mart Mannik1

1Department of Medicine, University of Washington, Seattle, Washington, USA

Correspondence: V Joyce Gauthier MD PhD, Department of Medicine, RG-28, University of Washington, Seattle, Washington 98195, USA.

Received 30 March 1987; Revised 20 July 1987; Re-revised 30 October 1987.

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References

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  3. Portis JL, Wikel SK, McAtee US: Technical methods: A simplified rapid method for purification of glomeruli. J Clin Pathol 32:406–409, 1979
  4. Gauthier VJ, Mannik M, Striker GE: Effect of cationized antibodies in preformed immune complexes on deposition and persistence in renal glomeruli. J Exp Med 156:766–777, 1982 | Article | PubMed | ChemPort |
  5. Haakenstad AO, Striker GE, Mannik M: The glomerular deposition of soluble immune complexes prepared with reduced and alkylated antibodies and with intact antibodies in mice. Lab Invest 35:293–301, 1976 | PubMed | ChemPort |
  6. Cook WF, Pickering GW: A rapid method for separating glomeruli from rabbit kidney. Nature 182:1103–1104, 1958 | Article | PubMed | ISI | ChemPort |
  7. Rytand DA: The number and size of mammalian glomeruli as related to kidney and to body weight, with methods for their enumeration and measurement. Am J Anat 62:507–520, 1938
  8. Mannik M, Gauthier VJ, Stapleton S, Agodoa LYC: Immune complexes with cationic antibodies deposit in glomeruli more effectively than cationic antibodies alone. J Immunol 38:4209–4217, 1987
  9. Salant DJ, Darby C, Couser WG: Experimental membranous nephropathy. Quantitative studies of glomerular immune deposit formation in isolated glomeruli and whole animals. J Clin Invest 66:71–81, 1980 | PubMed | ISI | ChemPort |

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