Laboratory Investgation

Kidney International (1986) 30, 62–67; doi:10.1038/ki.1986.151

Effects of the anaphylatoxin, C5a, on renal and glomerular hemodynamics in the rat

Juan C Pelayo, Dennis E Chenoweth, Tony E Hugli, Curtis B Wilson and Roland C Blantz

Departments of Medicine and Pathology, University of California, San Diego School of Medicine and Veterans Administration Medical Center, San Diego, and the Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California, USA

Correspondence: Roland C Blantz MD, Professor of Medicine, Veterans Administration Medical Center 111(H), 3350 La Jolla Village Drive, San Diego, California 92161, USA.

Received 21 January 1985; Revised 31 July 1985; Re-revised 21 November 1985.

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Abstract

Effects of the anaphylatoxin, C5a, on renal and glomerular hemodynamics in the rat. The effects of intrarenal infusion of the complement–derived anaphylatoxin, C5a, upon glomerular hemodynamics were examined in the Munich–Wistar rat, a strain with glomeruli on the kidney surface. Human C5a (1.5 microg/min) or vehicle was infused into the left renal artery for 12 min, and glomerular capillary (PG) and Bowman's space pressures, nephron plasma flow (SNPF) afferent and efferent arteriolar protein concentrations, nephron filtration rate (SNGFR) and the glomerular ultrafiltration coefficient (LpA) determined. Human C5a infusion resulted in a reduction in SNPF due to increased efferent arteriolar resistance, and PG increased which maintained SNGFR constant. LpA was numerically lower but not significantly decreased. Infusion of porcine C5ades Arg decreased glomerular filtration rate and renal blood flow. No polymorphonuclear leukocytes were observed within glomerular capillaries of C5a infused rats, and rat leukocytes did not exhibit receptors for human C5a infused. Renal artery infusion of either human C5a or porcine C5a resulted in renal hemodynamic alterations and, as documented for human C5a, effects of C5a upon renal vascular resistance can be added to the known effect of C5a on the polymorphonuclear leukocyte.

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