Laboratory Investgation

Kidney International (1986) 30, 56–61; doi:10.1038/ki.1986.150

Angiotensin II control of the renal microcirculation: Effect of blockade by saralasin

Michael Steinhausen, Helmut Kücherer, Niranjan Parekh, Stefan Weis, David L Wiegman and Karl-Rainer Wilhelm

First Institute of Physiology, University of Heidelberg, Heidelberg, Federal Republic of Germany, and Department of Physiology, University of Louisville, Louisville, Kentucky, U.S.A.

Correspondence: Dr M Steinhausen, I. Physiologisches Institut der Universitat Heidelberg, Im Neuenheimer Feld 326, D-6900 Heidelberg, Federal Republic of Germany.

Received 27 March 1984; Revised 26 June 1985; Re-revised 13 November 1985.

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Abstract

Angiotensin II control of the renal microcirculation: Effect of blockade by saralasin. The hydronephrotic rat kidney with intact circulation and innervation was split and spread out as a thin sheet in a tissue bath. The microvasculature was observed in vivo via television microscopy. We quantitated the effects of increasing concentrations (10-9 to 10-5 M) of saralasin (angiotensin II antagonist) applied locally in the tissue bath on microvascular diameters and on relative glomerular blood flow (measured using fluorescent labeled RBCs). Saralasin produced an increase in preglomerular diameters which was largest (37plusminus11%) in the interlobular artery (there was no dilation in the afferent arteriole near the glomerulus), an increase in postglomerular diameters which was largest (17plusminus4%) in the efferent arteriole near the glomerulus, and an increase in blood flow (19plusminus4%). If these types of findings would hold for the normal kidney, it would suggest a role for angiotensin II in the control of total renal blood flow, in the regional distribution of flow, and in the control of filtration fraction. We also made control micropressure measurements using the servo-nulling approach. Pressures measured were: afferent arteriole, 65plusminus5 mm Hg; intraglomerulus, 50plusminus5 mm Hg; and efferent arteriole, 19plusminus3 mm Hg. These data indicate that there is major vascular resistance near the glomerulus, especially in the efferent arteriole.

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