Laboratory Investigation

Kidney International (1985) 28, 504–512; doi:10.1038/ki.1985.157

Pathophysiology of the kidney in rats with Heymann nephritis

Marianna J Zamlauski-Tucker1, Judith B Van Liew1 and Bernice Noble1

1Departments of Microbiology and Physiology, State University of New York at Buffalo and Veterans Administration Medical Center, Buffalo, New York, USA

Correspondence: J B Van Liew, 151B, VA Medical Center, 3495 Bailey Avenue, Buffalo, New York 14215 USA

Received 7 November 1984; Revised 26 February 1985.

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Abstract

Pathophysiology of the kidney in rats with Heymann nephritis. Alterations in kidney function were assessed early in the course of Heymann nephritis that was induced in rats by immunization with Fx1A, an extract prepared from rat kidney cortex. Whole kidney and single nephron function were evaluated by clearance and micropuncture techniques. Kidney function was studied in stage 1 of Heymann nephritis, before the onset of proteinuria, and in stage 2, when antibodies are deposited along the brush border of proximal tubules. Although overall kidney function was similar in rats in stage 1 and normal controls, glucose reabsorption was somewhat depressed in the first part of the proximal convoluted tubule in stage 1. Both whole kidney and single nephron glomerular filtration rates were depressed in stage 2. Proteinuria in stage 2 was characterized by an increased albumin sieving coefficient, which resulted in an elevated excretion of albumin. Furthermore, several proximal tubule functions (glucose and fluid reabsorption and PAH extraction) were substantially depressed in stage 2. These findings demonstrate that immunological injury to the proximal tubules in stage 2 of Heymann nephritis produces a significant impairment of proximal function.

Physiopathologie du rein chez des rats atteints de néphrite de Heymann. Les altérations de la fonction rénale ont été mesurées précocement au cours d'une néphrite de Heymann, qui a été induite chez des rats par immunisation avec Fx1A, un extrait préparé à partir de cortex de rein de rat. La fonction rénale globale et néphronique individuelle a été évaluée par des techniques de clearance et de microponction. La fonction rénale a été étudiée au stade 1 de la néphrite de Heymann, avant l'apparition de la protéinurie, et au stade 2 lorsque les anticorps étaient déposés le long de la bordure en brosse des tubules proximaux. Bien que la fonction rénale globale soit identique chez des rats au stade 1 et chez les contrôles normaux, la réabsorption de glucose était quelque peu diminuée dans la première partie du tubule contourné proximal au stade 1. Les débits de filtration glomérulaire du rein entier et néphronique individuel étaient diminués au stade 2. La protéinurie, au stade 2, était caractérisée par une augmentation du coefficient de filtration de l'albumine, qui aboutissait à une élévation de l'excrétion d'albumine. De plus, les fonctions tubulaires proximales (réabsorption de glucose et de liquides et extraction du PAH) étaient substantiellement diminuées au stade 2. Ces résultats démontrent qu'une atteinte immunologique des tubules proximaux au stade 2 de la néphrite de Heymann induit une altération significative de la fonction proximale.

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