Laboratory Investigation

Kidney International (1984) 25, 404–410; doi:10.1038/ki.1984.31

Release of platelet-activating factor, slow-reacting substance, and vasoactive amines from isolated rat kidneys

Eduardo Pirotzky, Jocelyne Bidault, Claude Burtin, Marie Claire Gubler and Jacques Benveniste

Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 200, Université Paris-Sud, Clamart; INSERM Unité 203, Faculté Necker-Enfants Malades, Paris; and INSERM Unité 192, Hôpital Necker-Enfants Malades, Paris, France

Correspondence: Dr E Pirotzky, INSERM Unité 200, Université Paris-Sud, 32, rue des Carnets, 92140 Clamart, France

Received 10 September 1982; Revised 13 July 1983.

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Abstract

Release of platelet-activating factor, slow-reacting substance, and vasoactive amines from isolated rat kidneys. The present work brings the first evidence for the simultaneous release of Paf-acether (platelet-activating factor), slow-reacting substance (SRS), histamine, and serotonin from isolated rat kidneys stimulated with ionophore A 23187. However, with compound 48/80 we detected only SRS, histamine, and serotonin. Upon addition of antigen, kidneys from sensitized rats released Paf-acether and SRS of anaphylaxis. Paf-acether released in the perfusate was identified by its ability to aggregate aspirin-treated washed rabbit platelets in the presence of an ADP scavenger complex. It was also characterized by its inactivation by phospholipase A2, and it was eluted from high pressure liquid chromatography (HPLC) after 16 to 18 min, a retention identical to that of synthetic Paf-acether, that is, between sphingomyelin and lysophosphatidylcholine. The biological activity of SRS was detected after several purification steps including Amberlite XAD-2 and reverse phase HPLC (RP-HPLC). Kidney SRS exhibited a typical spasmogenic activity in isolated guinea-pig ileum preparation that was reversed by FPL 55712. When kidneys were incubated with [3H]arachidonic acid, radioactivity and biological activity comigrated in RP-HPLC with leukotrienes C and D. These results indicate that the kidney is capable of actively released inflammatory mediators.

Libération du platelet-active facteur, de la lent-réagit substance, et d'aminés vasoactives, à partir de reins isolés de rats. Nous avons mis en évidence la libération de Paf-acéther (platelet-active facteur), lent-réagit substance (SRS), histamine, et sérotonine par le rein de rat isolé et stimulé par du ionophore A 23187. Une stimulation au 48/80 induit seulement la libération de la SRS, histamine, et sérotonine. En présence de l'antigène, les reins de rats sensibilisés libèrent du Paf-acéther et de la SRS. Le Paf-acéther rénal a été caractérisé par sa capacité à agréger les plaquettes lavées de lapin en présence d'aspirine et du complexe creatine phosphate/créatine phosphokinase, son inactivation par la phospholipase A2 de pancréas, et son temps de rétention en chromatographic liquide à haute pression (HPLC) entre la sphingomyéline et la lysophosphatidylcholine, de 16 à 18 min, identique à celui du Paf-acéther synthétique. La SRS a été détectée après purification sur colonne d'Amberlite XAD-2 et par HPLC en phase réverse (RP-HPLC). Elle a été caractérisée par sa capacité à contracter l'iléon de cobaye en présence d'atropine et d'antihistaminique, contraction qui est relaxée spécifiquement par le FPL 55712, par son incorporation d'acide arachidonique tritié et l'élution en RP-HPLC de la radioactivité à des temps de rétention identiques à ceux des leukotriènes C et D synthétiques. Ces résultats démontrent que le rein est capable de libérer des médiateurs de l'inflammation.

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