Physiologic Perspectives from Isolated Tubule Studies

Kidney International (1982) 22, 498–506; doi:10.1038/ki.1982.203

Divalent anion transport in isolated renal tubules

Vincent W Dennis1 and Peter C Brazy1

1Division of Nephrology, Duke University Medical Center and Veterans' Administration Medical Center, Durham, North Carolina

Correspondence: Dr V W Dennis, Division of Nephrology, Duke University Medical Center, P.O. Box 3014, Durham, North Carolina 27710, USA

Received 15 June 1982.

Top

Abstract

Inorganic phosphate and sulfate are the major divalent anions in biological fluids at physiological pH. The isolated perfused renal tubule technique has been used to address a number of questions about their renal transport; the purpose of this review is to summarize this information. Initial studies addressed traditional questions about the direction and segmental localization of phosphate and sulfate transport but recent studies approach more fundamental questions about cellular mechanisms and interactions with metabolism. We will review these data and provide models that may be useful as targets for future studies.

Top

References

  1. Dennis VW, Woodhall PB, Robinson RR: Characteristics of phosphate transport in isolated proximal tubule. Am J Physiol 231:979–985, 1976
  2. McKeown JW, Brazy PC, Dennis VW: Intrarenal heterogeneity for fluid, phosphate and glucose absorption in the rabbit. Am J Physiol 237:F312–F318, 1979
  3. Rocha AS, Magaldi JB, Kokko JP: Calcium and phosphate transport in isolated segments of Henle's loop. J Clin Invest 59:975–983, 1977
  4. Dennis VW, Bello-Reuss E, Robinson RR: Response of phosphate transport to parathyroid hormone in segments of rabbit nephron. Am J Physiol 233:F29–F38, 1977 | PubMed | ChemPort |
  5. Peraino RA, Suki WN: Phosphate transport by isolated rabbit cortical collecting tubule. Am J Physiol 238:F358–F362, 1980
  6. Shareghi GR, Agus ZS: Phosphate transport in the light segment of the rabbit cortical collecting tubule. Am J Physiol 242:F379–F384, 1982
  7. Amiel C, Kuntziger H, Richet G: Micropuncture study of handling of phosphate by proximal and distal nephron in normal and parathyroidectomized rat. Evidence for distal reabsorption. Pflügers Arch 317:93–109, 1970
  8. Kuntziger H, Amiel C, Gaudebout C: Phosphate handling by the rat nephron during saline diuresis. Kidney Int 2:318–323, 1972
  9. Greger RF, Lang FC, Marchand GR, Knox FG: Site of renal phosphate reabsorption—micropuncture and microinfusion study. Pflügers Arch 369:111–118, 1977
  10. Brazy PC, Balaban RS, Gullans SR, Mandel LJ, Dennis VW: Inhibition of metabolism: Relative effects of arsenate on sodium, phosphate and glucose transport by the rabbit proximal tubule. J Clin Invest 66:1211–1221, 1980
  11. Brazy PC, McKeown JW, Harris RH, Dennis VW: Comparative effects of dietary phosphate, unilateral nephrectomy and parathyroid hormone on phosphate transport by the rabbit proximal tubule. Kidney Int 17:788–800, 1980
  12. Steele TH, DeLuca HF: Influence of dietary phosphorus on renal phosphate reabsorption in the parathyroidectomized rat. J Clin Invest 57:867–874, 1976 | PubMed | ChemPort |
  13. Trohler U, Bonjour J-P, Fleisch H: Inorganic phosphate homeostasis. Renal adaptation to the dietary intake in intact and thyroparathyroidectomized rats. J Clin Invest 57:264–273, 1976 | PubMed | ISI | ChemPort |
  14. Barrett PQ, Gertner JM, Rasmussen H: Effect of dietary phosphate on transport properties of pig renal microvillus vesicles. Am J Physiol 239:F352–F359, 1980 | PubMed | ISI | ChemPort |
  15. Kempson SA, Shah SV, Werness PG, Berndt T, Lee PH, Smith LH, Knox FG, Dousa TP: Renal brush border membrane adaptation to phosphorus deprivation: Effects of fasting versus low-phosphorus diet. Kidney Int 18:36–47, 1980 | PubMed | ISI | ChemPort |
  16. Dennis VW, Brazy PC: Sodium, phosphate, glucose, bicarbonate, and alanine interactions in the isolated proximal convoluted tubule of the rabbit kidney. J Clin Invest 62:387–397, 1978
  17. Gullans SR, Brazy PC, Dennis VW, Mandel LJ: Metabolic inhibitors: Effects on metabolism and transport in the proximal tubule. Am J Physiol 243:F133–F140, 1982 | PubMed |
  18. Hoffman N, Thees M, Kinne R: Phosphate transport by isolated renal brush border vesicles. Pflügers Arch 362:147–156, 1976
  19. Cheng L, Sacktor B: Sodium gradient-dependent phosphate transport in renal brush border membrane vesicles. J Biol Chem 256:1556–1564, 1981
  20. Tune BM, Burg M: Glucose transport by proximal renal tubules. Am J Physiol 221:580–585, 1971
  21. Brazy PC, Dennis VW: Characteristics of glucose-phlorizin interactions in isolated proximal tubules. Am J Physiol 234:F279–F286, 1978
  22. Barrett PQ, Aronson PS: Glucose and alanine inhibition of phosphate transport in renal microvillus membrane vesicles. Am J Physiol 242:F126–F131, 1982
  23. Kokko JP: Proximal tubule potential difference: Dependence on glucose, bicarbonate and amino acids. J Clin Invest 52:1362–1367, 1973
  24. Burg M, Green N: Bicarbonate transport by isolated perfused rabbit proximal convoluted tubules. Am J Physiol 233:F307–F314, 1977 | PubMed | ISI | ChemPort |
  25. Sacktor B, Cheng L: Sodium gradient-dependent phosphate transport in renal brush border membrane vesicles. J Biol Chem 256:8080–8084, 1981
  26. Berndt TJ, Knox FG: Effects of parathyroid hormone and calcitonin on electrolyte excretion in the rabbit. Kidney Int 17:473–478, 1980
  27. Chabardes D, Imbert M, Clique A, Montegut M, Morel F: Parathyroid hormone sensitive adenyl cyclase activity in different segments of the rabbit nephron. Pflügers Arch 354:229–239, 1975 | ChemPort |
  28. Dennis VW: Influence of bicarbonate on parathyroid hormoneinduced changes in fluid absorption by proximal tubule. Kidney Int 10:373–380, 1976
  29. Hamburger RJ, Lawson NL, Dennis VW: Effects of cyclic adenosine nucleotides on fluid absorption by different segments of proximal tubule. Am J Physiol 227:396–401, 1974
  30. Iino Y, Burg MB: Effects of parathyroid hormone on bicarbonate absorption by proximal tubules in vitro. Am J Physiol 236:F387–F391, 1979 | PubMed | ISI | ChemPort |
  31. McKinney TD, Myers P: PTH inhibition of bicarbonate transport by proximal convoluted tubules. Am J Physiol 239:F127–F134, 1980 | PubMed | ChemPort |
  32. Crane RK, Lipmann F: The effect of arsenate on aerobic phosphorylation. J Biol Chem 201:235–244, 1953 | PubMed | ISI | ChemPort |
  33. Rothstein A: Interactions of arsenate with the phosphate-transporting system of yeast. J Gen Physiol 46:1075–1085, 1963
  34. Levinson C: Phosphate transport in Ehrlich ascites tumor cells and the effect of arsenate. J Cell Physiol 79:73–78, 1972
  35. Kokko JP, Burg MB, Orloff J: Characteristics of NaCl and water transport in the renal proximal tubule. J Clin Invest 50:69–76, 1970
  36. Aronson PS: Identifying secondary active solute transport in epithelia. Am J Physiol 240:F1–F11, 1981 | PubMed | ISI | ChemPort |
  37. Beck JC, Sacktor B: The sodium electrochemical potential-mediated uphill transport of D-glucose in renal brush border membrane vesicles. J Biol Chem 253:5531–5535, 1978
  38. Hilden S, Sacktor B: Potential-dependent D-glucose uptake by renal brush border membrane vesicles in the absence of sodium. Am J Physiol 242:F340–F345, 1982
  39. Gullans SR, Brazy PC, Dennis VW, Mandel LJ: Mitochondrial substrates stimulate phosphate transport in the rabbit proximal tubule (abstract). Kidney Int 21:133, 1982
  40. Kempson SA, Colon-Otero G, Ou S-YL, Turner ST, Dousa TP: Possible role of nicotinamide adenine dinucleotide as an intracellular regulator of renal transport of phosphate in the rat. J Clin Invest 67:1347–1360, 1981 | PubMed |
  41. Brazy PC, Gullans SR, Mandel LJ, Dennis VW: Metabolic requirement for inorganic phosphate by the rabbit proximal tubule: Evidence for a Crabtree effect. J Clin Invest 70:53–62, 1982
  42. Ibsen KH: The Crabtree effect: A review. Cancer Res 21:829–841, 1961 | PubMed | ISI | ChemPort |
  43. Brazy PC, Gullans SR, Mandel LJ, Dennis VW: Influence of mitochondrial substrates on transport by phosphate limited rabbit proximal renal tubules (abstract). Clin Res 30:443A, 1982
  44. Brazy PC, Dennis VW: Sulfate transport in rabbit proximal convoluted tubules: Presence of anion exchange. Am J Physiol 241:F300–F307, 1981
  45. Lotspeich WD: Renal tubular reabsorption of inorganic sulfate in normal dog. Am J Physiol 151:311–318, 1947
  46. Cabantchick ZI, Rothstein A: The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives. J Membr Biol 10:311–330, 1974
  47. LaNoue KF, Schoolwerth AC: Metabolite transport in mitochondria. Annu Rev Biochem 48:871–922, 1979 | Article | PubMed | ISI | ChemPort |

Extra navigation

.
ADVERTISEMENT