Laboratory Investigation

Kidney International (1981) 20, 18–28; doi:10.1038/ki.1981.99

Dependency of proximal tubular fluid transport on the load of glomerular filtrate

D A Häberle, T T Shiigai*, G Maier, H Schiffl and J M Davis**

Physiologisches Institut der Universität München, Munich, West Germany

Correspondence: Dr D Häberle, Institute of Physiology, University of Munich, Pettenkoferstr. 12, 8000 Munich 2, West Germany

*Present address: Dept. of Medicine, Tokyo Medical and Dental Univ., 1, Yushima, Bunkyaku, Tokyo 113, Japan

**Present address: Dept. of Physiology, University of Melbourne, Parkville, Australia

Received 10 January 1980; Revised 16 September 1980.

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Abstract

Dependency of the proximal tubular fluid transport on the load of glomerular filtrate. In hydropenic rats, the reabsorption of glomerular filtrate by the proximal convoluted tubules was measured before and after reduction of its intratubular flow rate. Three different protocols were used. (1) In 26 tubules (14 rats), nephron glomerular filtration rate (SNGFR) was varied from 37.2 plusminus 7.3 to 20.4 plusminus 7.1 nl/min by microperfusing their loops of Henle at 0 to 5 nl/min and 40 nl/min, respectively. This 43% reduction of SNGFR was followed by a 36.0 plusminus 23.3% reduction of volume reabsorption rate (P < 0.001). Between both parameters a linear regression line can be calculated, which is given by y = 0.92x + 0.0017. (2) In 17 tubules (14 rats), SNGFR was altered again by feedback from 46.0 plusminus 9.7 to 28.8 plusminus 9.3 nl/min. The volume reabsorption from the first half of the proximal convoluted tubule was compared with the reabsorption in its late proximal segments, which were microperfused with proximal tubular fluid at a rate of 20 nl/min. The 36.8% reduction of SNGFR was followed by only a 28.2% reduction of volume reabsorption rate in the first half of the tubule. In the microperfused segments, however, reabsorption remained unaltered. (3) In 29 tubules (21 rats), at the midpoint of proximal convolutions, some of the tubule fluid was removed by a suction pump, and volume reabsorption rate in the late segments was compared with that in the early parts of this tubule, when SNGFR remained stable. The reduction of intratubular flow from 27.7 plusminus 8.5 to 14.7 plusminus 5.8 nl/min, which is 53% of control, was followed by a reduction of volume reabsorption rate in the late segment to 60.6% of control. Between both parameters a regression line was calculated, which is given by y = 0.76x plusminus 0.01. We conclude that the rate of volume reabsorption by the proximal tubule depends on its intratubular load of glomerular filtrate and, further, that this dependency accounts predominantly for the maintenance of glomerular tubular balance under conditions of hydropenia.

Dépendance du transport tubulaire proximal de liquide vis à vis de la charge de filtrat glomérulaire. La réabsorption de filtrat glomérulaire par les tubes contournés proximaux de rats hydropéniques a été mesurée avant et après réduction du débit intratubulaire. Trois protocoles différents ont été utilisés. (1) Dans 26 tubules (14 rats), la filtration glomérulaire des nephrons (SNGFR) a été modifiée entre 37,2 plusminus 7,3 et 20,4 plusminus 7,1 nl/min par la microperfusion de leurs anses de Henle de 0 à 5 et 40 nl/min, respectivement. Cette réduction de 43% de SNGFR a été suivie d'une réduction de 36,0 plusminus 23,3% du débit de réabsorption (P < 0,001). Une régression linéaire a été calculée entre les deux paramètres, y = 0,29* + 0,0017. (2) Dans 17 tubules (14 rats), SNGFR a été modifiée par feed back de 46,0 plusminus 9,7 à 28,8 plusminus 9,3 nl/min. La réabsorption dans la première moitié de tube contourné proximal a été comparée avec la réabsorption dans ses segments tardifs qui ont été microperfusés avec du liquide tubulaire proximal à un débit de 20 nl/min. La réduction de 36,8% de SNGFR a été suivie d'une réduction de 28,2% seulement du débit de réabsorption dans la première moitié du tubule. Dans les segments microperfusés, cependant, la réabsorption est restée inchangée. (3) Dans 29 tubules (21 rats), une partie du liquide tubulaire a été soustraite, au milieu de la partie contournée et le débit de réabsorption dans les segments tardifs a été comparé à celui des segments précoces, mesuré avec SNGFR stable. La réduction du débit intratubulaire de 27,7 plusminus 8,5 à 14,7 plusminus 5,8 nl/min, c'est à dire à 53% des valeurs contrôles, a été suivie d'une réduction du débit de réabsorption dans le segment tardif à 60,6% de la valeur contrôle. Une régression linéaire a été calculée entre les deux paramètres: y = 0,76x + 0,01. Il est conclu que le débit de réabsorption par le tube proximal dépend de sa charge intratubulaire en filtrat glomérulaire et que, de plus, cette dépendance rend compte, pour l'essentiel, du maintien de la balance glomérulotubulaire dans des conditions d'hydropénie.

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