Laboratory Investigation

Kidney International (1972) 2, 101–106; doi:10.1038/ki.1972.77

ATP content of rat kidney cortex slices: Relation to alpha-aminoisobutyric acid uptake

Claire Rea1 and Stanton Segal1

1Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia and the Department of Pediatrics, Medical School of the University of Pennsylvania, Philadelphia, Pennsylvania

Correspondence: Dr Stanton Segal, Department of Pediatrics, Childrens Hospital of Philadelphia, 1740 Bainbridge Street, Philadelphia, Pennsylvania 19146, U.S.A.

Received 17 March 1972; Accepted 12 May 1972.

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Abstract

ATP content of rat kidney cortex slices: Relation to alpha-aminoisobutyric acid uptake. ATP was measured in rat kidney cortex slices incubated in Krebs-Ringer bicarbonate buffer at 37° C and found to be 0.94 micromoles/g wet weight. The additions of various metabolic substrates were associated with both elevation and depression of this level. Octanoate produced the biggest decrease of ATP to 0.38 micromoles/g. Glucose, malate and succinate did not alter the level; acetate increased the level slightly. However, the greatest increase was produced by the addition of ATP to the medium (1.37 micro moles/g). Anaerobiosis and amytal, dinitrophenol, oligomycin and maleic acid additions were all associated with markedly lowered tissue ATP. The ATP content of tissue slices stored at 4° C for 24 to 72 hr was about half the value of fresh tissue slices. The relationship of ATP level to steady state alpha-aminoisobutyric acid uptake was examined. Normal amino acid uptake was seen over a wide range of ATP levels (0.38 to 1.37 micromoles/g). There was no direct correlation of ATP concentration with amino acid accumulation. The same decreased ATP level might be associated with normal uptake or depressed uptake depending on the means of lowering ATP. Depletion of ATP by anaerobiosis or metabolic inhibitors was, however, consistently associated with decreased amino acid uptake.

Contenu en ATP des tranches de cortex rénal de rat: Relation avec le prélèvement d'acide alpha-aminoisobutyrique. L'ATP a été mesurée dans des tranches de cortex rénal de rat incubées dans du tampon Krebs-Ringer bicarbonate à 37° C, la valeur obtenue est de 0.94 micromoles/g de poids humide. Les additions de divers substrats métaboliques ont augmenté ou diminué ces valeurs. L'octanoate a produit la baisse la plus importante de l'ATP à 0.38 micromoles/g. Le glucose, le malate et le succinate n'ont pas entraîné de modification. L'acétate l'a augmenté légèrement et l'augmentation la plus importante a été produite par l'addition d'ATP elle-même au milieu (1.37 micromoles/g). L'anaérobiose et l'addition d'amytal, de dinitrophénol, d'oligomycine et d'acide maléique ont été associés avec une réduction nette de l'ATP tissulaire. Le contenu en ATP de tranches de tissu conservées a 4° C pendant 24 à 72 heures était d'environ la moitié de celui des tranches de tissu frais. La relation entre le contenu en ATP et le prélèvement d'acide alpha-aminoisobutyrique a été étudiée. Le prélèvement normal de l'amino acide a été observé dans un large éventail de valeurs de l'ATP (0.38 à 1.37 micromoles/g). II n'a pas été constaté de corrélation directe entre la concentration d'ATP et l'accumulation d'aminoacide. La même concentration basse d'ATP peut être associée à un prélèvement normal ou diminué en fonction du moyen utilisé pour abaisser l'ATP. La déplétion de l'ATP par l'anaérobiose ou les inhibiteurs métaboliques a été, cependant, uniformément associée avec une diminution du prélèvement déamino acide.

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References

  1. Rosenberg L, Blair A, Segal S: The transport of amino acids by rat kidney cortex slices. Biochim Biophys Acta 54:479–488, 1961 | Article | PubMed | ISI | ChemPort |
  2. Rosenberg L, Downing S, Segal S: Competitive inhibition of dibasic amino acid transport in rat kidney. J Biol Chem 237:2265–2270, 1962 | PubMed | ChemPort |
  3. Rosenberg L, Berman M, Segal S: Studies of the kinetics of amino acid transport, incorporation into protein and oxidation in kidney cortex slices. Biochim Biophys Acta 71:664–685, 1963 | Article | PubMed | ISI | ChemPort |
  4. Fox M, Thier S, Rosenberg L, Segal S: Ionic requirements for amino acid transport in the rat-kidney-cortex slices. I. Influence of extracellular ions. Biochim Biophys Acta 79:167–176, 1964
  5. Rosenberg L, Segal S: Maleic acid induced inhibition of amino acid transport in rat kidney. Biochem. J 92:345–352, 1964
  6. Thier S, Fox M, Rosenberg L, Segal S: Hexose inhibition of amino acid uptake in rat kidney cortex slice. Biochim Biophys Acta 93:106–115, 1964
  7. Schwartzman L, Blair A, Segal S: Exchange diffusion of dibasic amino acids in rat kidney cortex slices. Biochim Biophys Acta 135:120–126, 1967
  8. Segal S, Schwartzman L, Bertoli D, Blair A: Dibasic amino acid transport in rat kidney cortex slices. Biochim Biophys Acta 135:127–135, 1967
  9. Schwartzman L, Blair A, Segal S: Effect of transport inhibitors on dibasic amino acid exchange diffusion in rat kidney cortex. Biochim Biophys Acta 135:136–145, 1967
  10. Their S, Blair A, Fox M, Segal S: The effect of extracellular sodium on the kinetics of AIB transport in rat kidney cortex slice. Biochim Biophys Acta 135:300–305, 1967
  11. Lowenstein L, Hummeler K, Smith I, Segal S: The effect of storage at 4° C on amino acid transport by rat kidney cortex slices. Biochim Biophys Acta 150:416–423, 1968 | PubMed | ISI | ChemPort |
  12. Segal S, Crawhall JC: The characteristics of cysteine and cystine transport in the rat kidney. Proc Natl Acad Sci 59:231–237, 1968
  13. Segal S, Genel M, Smith I: The effect of storage at 4° C on alpha-methylglucoside transport by rat kidney cortex slices. J Lab Clin Med 72:778–785, 1968 | PubMed | ISI | ChemPort |
  14. Segal S, Smith I: Delineation of cystine and cysteine transport systems in rat kidney cortex by developmental patterns. Proc Natl Acad Sci 63:926–933, 1969
  15. Segal S, Rea C, Smith I: Separate transport systems for sugars and amino acids in developing rat kidney cortex. Proc Natl Acad Sci 68:372–376, 1971
  16. Needleman P, Passonneau JV, Lowry OH: Distribution of glucose and related metabolites in rat kidney. Am J Physiol 215:655–659, 1968
  17. Hems DA, Brosnan JT: Effects of Ischaemia on content of metabolites in rat liver and kidney in vivo. Biochem J 120:105–111, 1970 | PubMed | ISI | ChemPort |
  18. Weidemann MJ, Hems DA, Krebs HA: Effects of added adenine nucleotides on renal carbohydrate metabolism. Biochem J 115:1–10, 1969
  19. Genel M, Rea C, Segal S: Transport interaction of sugars and amino acids in mammalian kidney. Biochim Biophys Acta 241:779–788, 1971
  20. Nagata N, Rasmussen H: Renal gluconeogenesis: Effects of Ca2+ and H+. Biochim Biophys Acta 215:1–16, 1970
  21. Weidemann MJ, Hems DA, Krebs HA: Effects of adenine nucleotides on renal metabolism. Nephron 6:282–296, 1969
  22. Weidemann MJ, Krebs HA: The fuel of respiration of rat kidney cortex. Biochem J 112:149–166, 1969 | PubMed |
  23. Fugimoto M, Nash FD, Kessler RH: Effects of cyanide, Qo and dinitrophenol on renal sodium reabsorption and oxygen consumption. Am J Physiol 206:1327–1332, 1964
  24. Urbaitis BK, Kessler RH: Concentration of adenine nucleotide compounds in renal cortex and medulla. Nephron 6:217–223, 1969
  25. Kramer HJ, Gonick HC: Experimental Fanconi syndrome. 1. Effect of maleic acid on renal cortical Na-K-ATPase activity and ATP levels. J Lab Clin Med 76:799–807, 1970
  26. States B, Holtzapple P, Rosenhagen M, Segal S: Kidney Internatl, in press
  27. Banay-Schwartz M, Piro L, Lajtha A: Relationship of ATP levels to amino acid transport in slices of mouse brain. Arch Biochem Biophys 145:199–210, 1971

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