Laboratory Investigation

Kidney International (1979) 15, 457–462; doi:10.1038/ki.1979.61

Effects of 24-hour unilateral ureteral obstruction on glomerular hemodynamics in rat kidney

Antonio Dal Canton1, Ariberto Corradi1, Rodolfo Stanziale1, Gianfranco Maruccio1 and Luigi Migone1

1Seconda Clinica Medica, University of Parma, Parma, Italy

Correspondence: Dr A Dal Canton, Divisione di Nefrologia Medica, Seconda Facoltà di Medicina Università, via S. Pansini, 80131 Napoli, Italia

Received 27 April 1978; Revised 4 October 1978.

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Abstract

Effects of 24-hour unilateral ureteral obstruction on glomerular hemodynamics in rat kidney. Glomerular hemodynamics were studied, by micropuncture, in Munich-Wistar rats submitted to 24-hour unilateral ureteral ligation (UUL). Glomerular capillary pressure (PG), intratubular pressure (PT) and pressure in the first-order peritubular capillaries (EAP) were measured with a servonulling device. Single nephron filtration fraction (SNFF) was calculated from arterial and peritubular blood protein concentration. SNGFR was both measured by conventional micro-puncture techniques and calculated from efferent arteriole blood flow (EABF) and SNFF. Afferent arteriole blood flow (AABF) and resistance of afferent (Ra) and efferent (Re) arterioles were calculated. Measurements were repeated 1 to 2 hours after the release of the ureter. Sham-operated rats were used as control. UUL caused a marked increase in Ra (from 4.9 plusminus [SD] 2.4 to 12.7 plusminus 5.1 dynes/sec/cm-5). The fall in SNGFR (from 111.9 plusminus [SD] 23.9 to 34.4 plusminus 23.1 nl/min/kg body wt) was secondary to a decrease in both PG and AABF. A further increase in Ra (16.0 plusminus 6.7 dynesdotsecdotcm-5) occurred after releasing the ureter. SNGFR, however, was unaltered (33.7 plusminus 16.6 nl/min/kg body wt) since PG decreased parallel to PT, but AABF did not significantly change. Conclusion. Ureteral obstruction determines, in 24 hours, a marked cortical ischemia that is not promptly reversed by ureteral release.

Effets d'une obstruction urétérale unilatérale sur l'hémodynamique glomérulaire dans le rein de rat. L'hémodynamique glomérulaire a été étudiée par microponctions, chez des rats Munich-Wistar soumis à 24 heures de ligature unilatérale de l'uretère (UUL). La pression capillaire glomérulaire (PG), la pression intratubulaire (PT), et la pression dans les capillaires péritubulaires de premier ordre (EAP) ont été mesurées au moyen d'un dispositif à zéro asservi. La fraction de filtration des néphrons individuels (SNFF) a été calculée à partir des concentrations de protéines dans le sang artériel et péritubulaire. SNGFR a été mesuré à la fois par les techniques usuelles de microponction et calculé à partir du débit artériolaire efférent (EABF) et de SNFF. Le débit artériolaire afférent (AABF) et les résistances des artérioles afférente (Ra) et efférente (Re ont été calculés. Les mesures ont été répétées une à deux heures après la libération de l'uretère. Des rats ayant subi un simulacre d'opération ont été utilisés comme contrôles. UUL a déterminé une augmentation importante de Ra (de 4,9 plusminus [SD] 2,4 à 12,7 plusminus 5,1 dynes/sec/cm-5). La diminution de SNGFR (de 119,9 plusminus [SD] 23,9 à 34,4 plusminus23,1 nl/min/kg poids corporel) a été secondaire à la diminution à la fois de PG et de AABF. Une augmentation supplémentaire de Ra (16,0 plusminus 6,7 dynesdotsecdotcm-5) est survenue après la libération de l'uretère. SNGFR, toutefois, n'a pas été affecté (33,7 plusminus 16,6 nl/min/kg poids corporel) du fait que PG a diminué parallèlement à PT alors que AABF n'a pas changé significativement. En conclusion l'obstruction urétérale détermine, en 24 heures, une ischémie corticale importante qui n'est pas rapidement réversible après la libération de l'uretère.

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