Laboratory Investigation

Kidney International (1972) 1, 78–88; doi:10.1038/ki.1972.11

Enzyme activities of the single juxtaglomerular apparatus in the rat kidney

Pierre Granger1, Herbert Dahlheim1 and Klaus Thurau1

1Department of Physiology, University of Munich, Munich, Germany

Correspondence: Dr Klaus Thurau, Physiologisches Institut der Universität München, 8 München 2, Pettenkoferstr. 12, Germany.

Received 11 August 1971; Revised 27 September 1971.

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Abstract

Enzyme activities of the single juxtaglomerular apparatus in the rat kidney. The single juxtaglomerular apparatus (JGA) can be easily microdissected according to the following procedure: the renal vascular tree is filled with Microfil, its progression through the vascular system is stopped by clamping the renal pedicle, and the kidney is then snap-frozen immediately. Once freeze-dried, single JGA's can be dissected under the stereomicroscope. Microfil injection permits precise identification of the afferent arteriole of the glomerulus. Converting enzyme activity was measured in single JGA's and found to be present. Angiotensinase activity was analysed in different structures of the rat kidney. The juxtamedullary JGA contained more angiotensinase activity than did the superficial JGA. Angiotensinase activity, in both the superficial and juxtamedullary JGA, was reduced approximately 50% when EDTA was added to the incubation mixture. Renin activity was measured in superficial and juxtamedullary JGA's of rats maintained on a standard diet, or rats that were sodium-loaded or sodium-deprived. Although the superficial JGA of sodium-deprived rats exhibited a higher mean renin activity than did the juxtamedullary JGA, this difference was not significant statistically. The superficial and juxtamedullary JGA of sodium-deprived rats was found to have significantly higher renin activity than that of the superficial or juxtamedullary JGA in sodium-loaded rats.

Activites enzymatiques de l'appareil juxtaglomérulaire isolé du rein de rat. L'appareil juxtaglomérulaire isolé (JGA) peut être facilement microdisséqué selon la procédure suivante: l'arbre vasculaire rénal est rempli avec du Microfil, et sa progression dans le système vasculaire est arrêtée par clampage du pédicule rénal et les reins sont alors immédiatement congelés. Une fois, congelés, des JGA isolés peuvent être disséqués sous le stéréomicroscope. L'injection de Microfil permet d'identifier de façon précise l'artériole afférente du glomérule. L'activité de l'enzyme de conversion a été mesuréedans des JGA isolés et sa présence a été confirmée. L'activité de l'angiotensinase a été analysée dans différentes structures du rein de rat. Les JGA juxtamédullaires contenaient plus d'activité d'angiotensinase que les JGA superficiels. L'activité de l'angiotensinase dans les JGA superficiels aussi bien que juxtamédullaires a été réduite de 50% approximativement lorsque de l'EDTA était ajouté au milieu d'incubation. L'activité rénine a été mesurée dans les JGA superficiels et juxtamédullaires de rats maintenus sous diète normale ou de rats surchargés ou déplétés en sodium. Bien que les JGA superficiels des rats déplétés en sodium contenaient une plus grande activité moyenne en rénine que les JGA juxtamédullaires, cette différence n'était pas significative statistiquement. Il s'est avéré que le contenu en activité rénine des JGA superficiels et juxtamédullaires des rats déplétés en sodium était significativement plus élevé que les JGA superficiels et juxtamédullaires des rats surchargés en sodium.

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