FIGURE 3

Pre-treatment—PGP9.5 and SP expression. (a) PGP 9.5-positive epidermal and perifollicular nerves, as well nerves highlighting eccrine glands and the arrector pili muscle, are seen. (b) SP staining nerves and globule-like extra neuronal structures are present in the dermis. (c) Colocalization of PGP 9.5 (green) and SP (red) reveals some SP-containing nerve fibers throughout the scalp skin, but the majority of expression is within the vasculature (data not shown). Each picture is a projection of 46 optical sections taken at 1-micron intervals. Scale bar=100 microns.