FIGURE 2

Pre-treatment—PGP 9.5 and CGRP expression. (a) PGP 9.5-positive subepidermal nerves extend from this plexus into the epidermis, and perifollicular nerves are present in a basket-weave pattern. (b) CGRP-positive nerves can be found in the subepidermal nerve plexus and epidermis and as a network of nerves around a miniaturized hair follicle. (c) Colocalization of PGP 9.5 (green) and CGRP (red) in several subepidermal and perifollicular nerves is present. Each picture is a projection of 36 optical sections taken at 2-micron intervals. Scale bar=100 microns.