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Substantial Sex-Dependent Differences in the Response of Human Scalp Hair Follicles to Estrogen Stimulation In Vitro Advocate Gender-Tailored Management of Female Versus Male Pattern Balding
Franziska Conrad, Ulrich Ohnemus, Enikö Bodo, Tamas Biro, Birte Tychsen, Bernhard Gerstmayer, Andreas Bosio, Thomas Schmidt-Rose, Silke Altgilbers, Albrecht Bettermann, Matthias Saathoff, Wilfried Meyer and Ralf Paus
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Comparison of the steady-state levels for estrogen receptor-
and -
transcripts in male and female scalp hair follicles by QT-PCR after 17-estradiol(E2)-stimulation in vitro. QT-PCR for ER
(blue) and ER
(green) (Assay-on demand, Applied Biosystems, Hs 00174860, Hs00230957) of total RNA from male and female frontotemporal scalp hair follicles (organ-cultured 48 hours), comparing control and E2-treated (10nM). No significant differences were given after normalizing to the internal standard GAPDH.
SEM
Table S1 (pdf 143K)
Gene expression ratios of female human scalp hair follicles after 17-estradiol(E2)-stimulation in vitro Using a commercial skin focus chip (microarray), over 1300 genes were screened for E2-dependence. The modulated genes are given with the standard deviation of the four replicate ratios of signal and background.
Table S2 (pdf 139K)
Gene expression ratios of male human scalp hair follicles after 17-estradiol(E2)-stimulation in vitro. Using a commercial skin focus chip (microarray), over 1300 genes were screened for E2-dependence. The modulated genes are given with the standard deviation of the four replicate ratios of signal and background.
