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FROM:
Analysis of Cultured Human Melanocytes Based on Polymorphisms within the SLC45A2/MATP, SLC24A5/NCKX5, and OCA2/P Loci
Anthony L Cook, Wei Chen, Amy E Thurber, Darren J Smit, Aaron G Smith, Timothy G Bladen, Darren L Brown, David L Duffy, Lorenza Pastorino, Giovanna Bianchi-Scarra, J Helen Leonard, Jennifer L Stow and Richard A Sturm
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Quantitation of melanocytic protein levels by pigmentation genotype. Quantitation of TYRP1, DCT, MART1, MITF and GAPDH protein levels (a–e respectively) after normalisation to IFA protein levels for MATP (left), NCKX5 (center) or OCA2 (right) polymorphisms respectively. Values shown are the mean 
SEM of all strains assayed for each genotype. Statistical tests were performed comparing samples for each SNP. ***p<0.001.
Figure S2 (pdf 36K)
Melanocytic mRNA expression in QF melanocyte strains based on genotype. Quantitative real-time PCR was employed to determine mRNA levels of TYRP1, DCT and SILV (a–c respectively) in individual QF strains. For each QF strain, melanocytic mRNA levels were normalised to 18S ribosomal RNA levels. Errors bars show mean SEM of 3 samples of each QF strain. In d–f, the data presented in a-c was averaged according to MATP-374 (left column), NCKX5-111 (middle column) or rs12913832 (right column) genotypes. Values shown are the mean SEM of all strains assayed for each genotype.
Table S1 (pdf 86K)
Genotype frequencies in Queensland Foreskin samples
Table S2 (pdf 94K)
Summary of p values for cellular analysis of pigmentation.
Table S3 (pdf 75K)
Distribution of pigmentation phenotypes among genotyped Southern European sample.
