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SIRT1 Promotes Differentiation of Normal Human Keratinocytes
Gil Blander, Anupama Bhimavarapu, Thomas Mammone, Daniel Maes, Keith Elliston, Christian Reich, Mary Steidl Matsui, Leonard Guarente and Joseph Jorge Loureiro
BACK TO ARTICLEDownload pluginsSupplementary Figures S1 (pdf 14K)
Causal model for SIRT1-induced keratinocyte differentiation. Four major hypotheses are indicated for the SIRT1 overexpressing cells and discussed in detail in the results section.
Supplementary Figures S2 (pdf 16K)
Causal model for SIRT1-induced keratinocyte differentiation. Seven major hypotheses are indicated for the SIRT1 RNAi cells and discussed in detail in the results section. Yellow and blue boxes represent predicted increase and decrease, respectively. Green and red boxes represent observed increases and decrease in RNA abundance respectively. Lines with arrowheads indicate causal activation; lines with bars indicate causal inhibition. The direction of the arrow in boxes indicates whether a process, hypothesis or observation shows an increase or decrease following SIRT1 overexpression or RNAi.
Supplementary Figures S3 (pdf 17K)
Cell cycle hypothesis in the SIRT1 RNAi cells. As in Figure 2. (a) In the SIRT1 overexpressing cells. (b) In the SIRT1 RNAi cells. Diagram of RNA state changes consistent with or contrary to the indicated hypothesis. Green—observed increase in RNA expression of a given gene; red—observed decrease in RNA expression of a given gene; yellow—hypothesized increase in biological processes or protein activity; blue—hypothesized decrease in biological processes or protein activity. "+" symbolizes causal activation; "-" symbolizes casual inhibition; "+" and "-" nodes are supported by published findings supporting the causal assertion between the hypothesis and the RNA state change. An "X" over an observed RNA expression change indicates a contradiction (the direction of the observed RNA expression change is inconsistent with the hypothesis it is connected to). General note: In figures where gene expression is depicted, expression is noted by "exp" and the NCBI gene symbol is in parentheses. For example, exp(KRT10) indicates a change in keratin 10 expression. In addition, placement within a particular color indicates whether the change in expression was observed to increase (green) or observed to decrease (red). Also predicted increase (yellow) or predicted decrease (blue) may also be indicated.
