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Journal of Investigative Dermatology (1992) 99, 887–891; doi:10.1111/1523-1747.ep12614896

The Core Protein of Epican, a Heparan Sulfate Proteoglycan on Keratinocytes, Is an Alternative Form of CD44

Lisa C Kugelman1,2, Subinay Ganguly3, John G Haggerty1,2, Sherman M Weissman3 and Leonard M Milstone2

  1. 1Dermatology Service, VA Medical Center, West Haven
  2. 2Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut, U.S.A.
  3. 3Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, U.S.A.

Received 20 March 1992; Accepted 26 May 1992.

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Abstract

Epican, a heparan sulfate proteoglycan, was recently identified on the surface of keratinocytes with the aid of a monoclonal antibody to its core protein. Using that antibody to screen a human keratinocyte cDNA library, a clone encoding the entire epican core protein was selected and sequenced. The core protein of epican is a form of CD44. The deduced protein sequence of 699 amino acids has a novel 339 amino acid domain inserted into the proximal extracellular domain of the standard, leukocyte form of CD44. The additional domain adds a number of potential N- and O-linked glycosylation sites and two proteolysis sites to this form of CD44.

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