Original Article

Journal of Investigative Dermatology (1991) 97, 478–482; doi:10.1111/1523-1747.ep12481515

Inhibition of Langerhans Cell ATPase and Contact Sensitization by Lanthanides—Role of T-Suppressor Cells

Stefan Gruner1, Wolfgang Diezel2, Dirk Strunk1, Rolf Eckert3, Werner Siems4 and Grant J Anhalt5

  1. 1Department of Dermatology, The Humboldt University, Berlin, Germany
  2. 2Department of Dermatology, University of Rostock, Rostock, Germany
  3. 3Department of Immunology, The Humboldt University, Berlin, Germany
  4. 4Department of Biochemistry, The Humboldt University, Berlin, Germany
  5. 5Department of Dermatology, The Johns Hopkins University, Baltimore, Maryland, U.S.A.

Received 13 October 1989; Accepted 26 April 1991.

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Abstract

Lanthanides are rare earths, elements 55–71 in the periodic table, that are of interest in biologic systems as isomorphic competitors for calcium binding sites. Lanthanides were tested for their inhibitory influence on the Ca++/Mg++-dependent ATPase of epidermal langerhans cells in vitro, and on the immunologic function of Langerhans cells in vivo. The trivalent ions of lanthanides, lanthanum and cerium completely inhibited the ATPase staining of Langerhans cells in vitro. When mice were sensitized with dinitrofluorobenzene on skin sites pretreated with topical lanthanum chloride, and challenged on untreated ear skin, a markedly reduced contact hypersensitivity response was observed. This hyporesponsiveness was found to be antigen specifics and could be passively transferred to naive syngeneic animals recipients by CD4- CD8+ spleen cells. These results suggest that inhibition of the epidermal Langerhans cell surface ATPase by application of topical lanthanum and the induction of antigen-specific immunologic tolerance may be related events.

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References

  1. Silberberg, I, Baer, RL, Rosenthal, SA, Thorbecke, GJ, Berezowsky, V: Dermal and intravascular Langerhans cells at sites of passively induced contact sensitivity. Cell Immunol 1975 18: 435–453,  | Article | PubMed | ISI | ChemPort |
  2. Stingl, G, Katz, SI, Clement, L, Green, I, Shevach, EM: Immunological functions of Ia bearing epidermal Langerhans cells. J Immunol 1978 121: 2005–2013,  | PubMed | ISI | ChemPort |
  3. Thorbecke, GJ, Silberberg-Sinakin, I, Flotte, IJ: Langerhans cells as macrophages in skin and lymphoid organs. J Invest Dermatol 1980 75: 32–43,  | Article | PubMed | ChemPort |
  4. Schmitt, D, Dezutter-Dambuyant, C, Faure, M, Thivolet, J: Recent advances on ultrastructural immunocytochemistry of epidermal Langerhans cells. Br J Dermatol 1985 113: 2–9,  | PubMed |
  5. Murphy, GF: Cell membrane glycoproteins and Langerhans cells. Perspectives in Pathology 1985 13: 103–112,
  6. Wolff, K, Winkelmann, RK: Ultrastructural localization of nucleoside triphosphatase in Langerhans cells. J Invest Dermatol 1967 18: 50–54,
  7. Chaker, MB, Tharp, MD, Bergstresser, PR: Rodent epidermal Langerhans cells demonstrate greater histochemical specificity for ADP than for ATP and AMP. J Invest Dermatol 1984 82: 496–500,  | Article | PubMed | ISI | ChemPort |
  8. MacKenzie, IC, Squier, CA: Cytochcmical identification of ATPase positive Langerhans cells of mouse epidermis. Br J Dermatol 1975 92: 523–533,  | PubMed | ISI | ChemPort |
  9. Tsien, RW, Hess, P, McCleskey, EW, Rosenberg, RL: Calcium channels: mechanisms of selectivity, permeation and block. Annu Rev Biophys Biophys Chem 1987 16: 265–290,  | Article | PubMed | ISI | ChemPort |
  10. Lansman, JB, Hess, P, Tsein, R: Blockade of current through single calcium channels by Cd++,Mg++, and Ca++. J Gen Physiol 1986 88: 321–347,  | Article | PubMed | ChemPort |
  11. Evan, CH: Interesting and useful biochemical properties of lanthanides. Trends Biochem Sci 1983 8: 445–449,
  12. Diezel, W, Gruner, S, Diaz, L, Anhalt, GJ: Suppression of contact hypersensitivity by calcium transport inhibitors lanthanum and diltiazem. J Invest Dermatol 1989 93: 322–326,  | Article | PubMed | ISI | ChemPort |
  13. Luterbacher, S, Schatzmann, HJ: The site of action of La+++ in the reaction cycle of the human red cell membrane Ca++-pump ATPase. Experientia 1983 39: 311–312,  | Article | PubMed | ChemPort |
  14. Quist, EE, Roufogalis, BD: Determination of the stoichiometry of the calcium pump in human erythrocytes using lanthanum as a selective inhibitor. FEES Lett 1975 50: 135–139,  | Article | ChemPort |
  15. Elmets, CA, Bergstresser, PR, Tigerlaar, RE, Wood, PJ, Streillein, JW: Analysis of the mechanism of unresponsiveness produced by haptens painted on skin exposed to low dose ultraviolet radiation. J Exp Med 1983 158: 781–794,  | Article | PubMed | ISI | ChemPort |
  16. Kripke, ML, Morison, WL: Modulation of immune function by ultraviolet radiation. J Invest Dermatol 1985 85: 62,
  17. Aberer, W, Schuler, G, Stingl, G, Hönigsmann, H, Wolff, K: Ultraviolet light depletes surface markers of Langerhans cells. J Invest Dermatol 1981 76: 202–210,  | Article | PubMed | ISI | ChemPort |
  18. Iacobelli, D, Hashimoto, K: Effect of ultraviolet radiation on guinea pig epidermal Langerhans cell cytomembrane. Photodermatol 1985 2: 132–141,  | PubMed | ChemPort |
  19. Granstein, RD: Epidermal I-J bearing cells are responsible for transferable suppressor cell generation after immunization of mice with ultraviolet radiation treated epidermal cells. J Invest Dermatol 1985 84: 206–209,  | Article | PubMed | ChemPort |
  20. Granstein, RD, Askari, M, Whitaker, D, Murphy, GF: Epidermal cells in activation of suppressor lymphocytes: further characterization. J Immunol 1987 138: 4055–4062,  | PubMed | ISI | ChemPort |
  21. Hanau, D, Fabre, M, Schmitt, DA, Stampf, JL, Garaud, JC, Bieber, T, Grosshans, E, Benezra, C, Cazenave, JP: Human epidermal Langerhans cells internalize by receptor mediated endocytosis T6 (Cd1 "Na1/34") surface antigen: Birbeck granulas are involved in the intracellular traffic of the T6 antigen. J Invest Dermatol 1987 89: 172–177,  | Article | PubMed | ISI | ChemPort |

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