Original Article

Journal of Investigative Dermatology (1991) 97, 405–409; doi:10.1111/1523-1747.ep12480983

Disparate Antioxidant Enzyme Activities in Cultured Human Cutaneous Fibroblasts, Keratinocytes, and Melanocytes

Joseph J Yohn1,2, David A Norris1,2, David G Yrastorza1, Irene J Buno1, Jonathan A Leff3,4, Steven S Hake1 and John E Repine3,4

  1. 1Department of Dermatology, University of Colorado, School of Medicine, Denver, Colorado, U.S.A.
  2. 2Dermatology Service, Department of Veterans Affairs Medical Center, Denver, Colorado, U.S.A.
  3. 3Department of Medicine,University of Colorado, School of Medicine, Denver, Colorado, U.S.A.
  4. 4The Webb-Warning Lung Institute, University of Colorado, School of Medicine, Denver, Colorado, U.S.A.

Received 5 March 1991; Accepted 9 May 1991.

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Abstract

Antioxidant enzyme activities of cultured human foreskin fibroblasts, keratinocytes and melanocytes from healthy black and Caucasian donors were measured and compared. Fibroblasts had more (p < 0.05) peroxidase, catalase, glutathione peroxidase and superoxide dismutase activity than keratinocytes. Keratinocytes had more (p < 0.05) peroxidase, catalase, glutathione peroxidas, and superoxide dismutase activity than melanocytes. No differences in antioxidant enzyme activities were observed between the cells of any type taken from black or Caucasian people. Antioxidant enzyme activities may affect resistance to damage by oxidants induced by ultraviolet radiation and inflammation.

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