Original Article

Journal of Investigative Dermatology (1990) 95, 543–547; doi:10.1111/1523-1747.ep12504896

Sodium Lauryl Sulphate for Irritant Patch Testing- A Dose-Response Study Using Bioengineering Methods for Determination of Skin Irritation

Tove Agner and Jørgen Serup

Department of Dermatology, Rigshospitatet, Copenhagen, Denmark

Received 7 February 1990; Accepted 20 June 1990.

Top

Abstract

The dose-response relationship in patch testing with sodium lauryl sulphate (SLS) was studied. The irritant skin response was quantified by visual scoring as well as by the following noninvasive methods: measurement of transepidermal water loss (TEWL) by an evaporimeter, measurement of skin color by a colorimeter, measurement of superficial blood flow by laser Doppler flowmetry, and measurement of edema in the skin by ultrasound A-scan. Twelve volunteers were patch tested with 0.12, 0.25, 0.50, and 1.00% SLS, and the skin response was evaluated after 24 and 48 h, respectively. We found a statistically significant linear dose-response relationship between dose of SLS and skin response evaluated by measurement of TEWL, skin color, superficial blood flow, and edema. Statistical evaluation by regression analysis proved measurement of TEWL to be the method best suited overall for quantification in relation to patch testing with SLS, whereas colorimetry was found to be the least sensitive of the applied methods. Ultrasound A-scan was found to be a promising method for quantification of the inflammatory response, being consistently more sensitive than measurement of skin color.

Top

References

  1. Agner, T, Serup, J: Skin reactions to irritants assessed by noninvasive bioengineering methods. Contact Dermatitis 1989 20: 352–359,  | Article | PubMed | ISI | ChemPort |
  2. Nilsson, GE: On the measurement of evaporative water loss. Linköping University Medical Dissertations No. 48 1977 Linköping University,
  3. Pinnagoda, J, Tupker, RA, Agner, T, Serup, J: Guidelines for transepidermal water loss (TEWL) measurement. Contact Dermatitis 1990 22: 164–178,  | Article | PubMed | ISI | ChemPort |
  4. Robertson, AR: The CIE 1976 color difference formulas. Color Res Appl 1977 2: 7–11,
  5. Serup, J, Agner, T: Colorimetric quantification of erythema. Clin Exp Dermatol (in press)
  6. Tenland, T: On laser Doppler flowmetry. Methods and microvascular applications 1982 Linköping Medical Dissertations No. 136, Linköping University,
  7. Scrap, J: Localized scleroderma (Morphoea). Thickness of the sclerotic plaques as measured by 15 MHz pulsed ultrasound. Acta Derm Venereol (Stockh) 1984 64: 214–219,
  8. Edwards, C, Payne, PA: Ultrasonic velocities in skin components (abstr.). Presented at International Society of Bioengineering of the Skin Meeting 1984, November 9, Liege, Belgium
  9. Agner, T, Serup, J: Individual and instrumental variation in patch test reactions. Clin Exp Dermatol 1990 15: 29–33,  | Article | PubMed | ChemPort |
  10. Finney, DJ: Statistical Method in Biological Assay 1978 3rd Ed. London and High Wycombe, Charles Griffin & Co. Ltd.,
  11. Bruynzeel, DP, Ketel, WG, Scheper, RJ, von Blomberg-van der Flier, BME: Delayed time course of irritation by sodium lauryl sulphate: Observation on threshold reactions. Contact Dermatitis 1982 8: 236–239,  | Article | PubMed | ChemPort |
  12. Wilhelm, KP, Surber, C, Maibach, HI: Quantification of sodium lauryl sulfate irritant dermatitis in man. Comparison of four techniques: skin color reflectance, transepidermal water loss, laser Doppler flow measurement and visual scores. Arch Dermatol Res 1989 281: 293–295,  | Article | PubMed | ISI | ChemPort |
  13. Blanken, R, Valk, PGM, Nater, JP: Laser-Doppler flowmetry in the investigation of irritant compounds on human skin. Dermatosen 1986 34: 5–9,  | ChemPort |
  14. Tupker, R, Coenraads, PJ, Pinnagoda, J, Nater, JP: Baseline transepidermal water loss (TEWL) as a prediction of susceptibility to sodium lauryl sulphate. Contact Dermatitis 1989 20: 265–269,  | Article | PubMed | ChemPort |
  15. Agner, T, Serup, J: Transepidermal water loss and air convection. Contact Dermatitis 1990 22: 120–121,  | Article | PubMed | ChemPort |
  16. Serup, J, Staberg, B: Ultrasound for assessment of allergic and irritant patch test reactions. Contact Dermatitis 1987 17: 80–84,  | Article | PubMed | ISI | ChemPort |
  17. Nilsson, GE, Otto, U, Wahlberg, JE: Assessment of skin irritancy in man by laser Doppler flowmetry. Contact Dermatitis 1982 8: 401–406,  | Article | PubMed | ChemPort |
  18. Staberg, B, Serup, J: Allergic and irritant skin reactions evaluated by laser Doppler flowmetry. Contact Dermatitis 1988 18: 40–45,  | Article | PubMed | ChemPort |
  19. Babulak, SW, Rhein, LD, Scala, DD, Simion, FA, Grove, GL: Quantitation of erythema in a soap chamber test using the Minolta Chroma (reflectance) Meter: Comparison of instrumental results with visual assessments. J Soc Cosmet Chem 1986 37: 475–477,
  20. Westerhof, W, Kammeijer, A: Quantification of UV-induced erythema with 2 portable computer controlled chromameter. Photodermatology 1986 3: 310–314,  | PubMed | ChemPort |
  21. Agner, T, Serup, J: Seasonal variation in irritant contact dermatitis. Br J Dermatol 1989 121: 323–328,  | PubMed | ISI | ChemPort |
  22. Frosch, P: Hautirritation und empfindliche Haut. Grosse Verlag Berlin, 1985, pp 18–20,

Extra navigation

.
ADVERTISEMENT