Original Article

Journal of Investigative Dermatology (1990) 94, 108s–111s; doi:10.1111/1523-1747.ep12876061

The Use of Human T-Lymphocyte Clones to Study T-Cell Function in Allergic Contact Dermatitis to Urushiol

Richard S Kalish

Department of Dermatology, University of Minnesota, Minneapolis, Minnesota, U.S.A.

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Abstract

Allergic contact dermatitis to poison ivy (Toxicodendron radicans) is believed to be mediated by T lymphocytes specific for the hapten urushiol. Activated T lymphocytes may produce pathology by a variety of mechanisms including direct cytotoxicity, production of lymphokines, recruitment of non-specific effector cells, non-specific cytotoxicity, and possibly autologous DR reactivity. The regulation and pathogenesis of this condition was studied by cloning and characterizing urushiol-specific T cells from the peripheral blood of patients with poison ivy dermatitis. Multiple CD8+ (T8+) urushiol-specific clones were derived. All clones that proliferated in response to a crude extract of T. radicans also proliferated in response to purified urushiol. Thus, urushiol appears to be the single immunogenic component of T. radicans resin. Pentadecylcatechol (PDC), which differs from urushiol only in the lack of unsaturated bonds in its lipophilic tail, stimulated only one of seven clones tested. This suggests that the double bonds in the C15 lipophilic tail of urushiol are required for antigenicity. Several of the CD8+ urushiol-specific clones suppressed pokeweed mitogen-induced IgG production in the presence of urushiol. Suppression was triggered specifically by urushiol and required MHC compatibility both for the antigen-presenting cells and the responding B cells. These suppressor clones were isolated from convalescent blood and may represent a mechanism for the termination of an allergic contact dermatitis.

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References

  1. Cantrell, DA, Smith, KA: Transient expression of interleukin 2 receptors. Consequences for T-cell growth. J Exp Med 1983 158: 1895–1911,  | Article | PubMed | ISI | ChemPort |
  2. Birnbaum, G, Kotilnek, L, Schwartz, M, Sternad, M: Spinal fluid lymphocytes responsive to autologous and allogeneic cells in multiple sclerosis and control individuals. J Clin Invest 1984 74: 1307–1317,
  3. Hoffmann, RM, Pape, GR, Rieber, P, Eisenburg, J, Döhrmann, J, Zachoval, R, Paumgartner, G, Riethmüller, G: Cytolytic T-cell clones derived from liver tissue of patients with chronic hepatitis B. Eur J Immunol 1986 16: 1635–1638,
  4. Canonica, GW, Caria, M, Bagnasco, M, Cosulich, ME, Giordano, G, Moretta, L: Proliferation of T8-positive cytolytic T lymphocytes in response to thyroglobulin in human autoimmune thyroiditis: Analysis of cell interactions and culture requirements. Clin Immunol Immunopathol 1985 36: 40–48,
  5. Preffer, FI, Colvin, RB, Leary, CP, Boyle, LA, Tuazon, TV, Lazarovits, AI, Cosimi, AB, Kurnick, JT: Two-color flow cytometry and functional analysis of lymphocytes cultured from human renal allografts: identification of a leu-2+3+ subpopulation. J Immunol 1986 137: 2823–2830,
  6. Mesler Muul, L, Spiess, PJ, Director, EP, Rosenberg, SA: Identification of specific cytolytic immune responses against autologous tumor in humans bearing malignant melanoma. J Immunol 1987 138: 989–995,
  7. Modlin, RL, Mehra, V, Wong, L, Fujimiya, Y, Chang, WC, Horwitz, DA, Bloom, BR, Rea, TH, Pattengale, PK: Suppressor T-lymphocytes from lepromatous leprosy skin lesions. J Immunol 1986 137: 2831–2834,  | PubMed | ChemPort |
  8. Ottenhoff, THM, Elferink, DG, Klatser, PR, de Vries, RRP: Cloned suppressor T-cells from a lepromatous leprosy patient suppress Mycobacterium leprae reactive helper T-cells. Nature 1986 322: 462–464,  | Article | PubMed | ChemPort |
  9. Moudlin, RL, Kato, H, Mehra, V, Nelson, EE, Xue-dong, F, Rea, TH, Pattengale, PK, Bloom, BR: Genetically restricted suppressor T-cell clones derived from lepromatous leprosy lesions. Nature 1986 322: 459–461,  | Article |
  10. Sinigaglia, F, Scheidegger, D, Garotta, G, Scheper, R, Pletscher, M, Lanzavecchia, A: Isolation and characterization of Ni-specific T-cell clones from patients with Ni-contact dermatitis. J Immunol 1985 135: 3929–3932,  | PubMed | ISI | ChemPort |
  11. Löfström, A, Wigzell, H: Antigen specific human T-cell lines specific for cobalt chloride. Acta Derm Venereol 1986 60: 200–206,
  12. Kligman, AM: Poison ivy (rhus) dermatitis. Arch Dermatol 1958 77: 149–180,  | ChemPort |
  13. Baer, H, Watkins, RC, Kurtz, AP, Byck, JS, Dawson, CR: Delayed contact sensitivity to catechols III. The relationship of side-chain length to sensitizing potency of catechols chemically related to the active principles of poison ivy. J Immunol 1967 99: 370–375,
  14. Byers, VS, Epstein, WL, Castagnoli, N, Baer, H: In vitro studies of poison oak immunity. I. In vitro reaction of human lymphocytes to urushiol. J Clin Invest 1979 64: 1437–1448,  | PubMed | ChemPort |
  15. Byers, VS, Castagnoli, N, Epstein, WL: In vitro studies of poison oak immunity. II. Effect of urushiol analogues on the human in vitro response. J Clin Invest 1979 64: 1449–1456,
  16. Dunn, IS, Liberato, DJ, Castagnoli, N, Byers, VS: Contact sensitivity to urushiol: role of covalent bond formation. Cell Immunol 1982 74: 220–223,  | Article | PubMed | ChemPort |
  17. Botham, PA, Rattray, NJ, Walsh, ST, Riley, EJ: Control of the immune response to contact sensitizing chemicals by cutaneous antigen presenting cells. Br J Dermatol 1987 117: 1–9,  | PubMed | ISI | ChemPort |
  18. Kupper, TS, Horowitz, M, Birchall, N, Mizutani, H, Coleman, D, McGuire, J, Flood, P, Dower, S, Lee, F: Hematopoietic, lymphopoietic and proinflammatory cytokines produced by human and murine keratinocytes. Ann NY Acad Sci 1988 548: 262–270,  | PubMed | ChemPort |
  19. Kupper, TS, Min, K, Sehgal, P, Mizutani, H, Birchall, N, Ray, A, May, L: Production of IL-6 by keratinocytes. Ann NY Acad Sci 1989 557: 454–465,  | PubMed | ChemPort |
  20. Kupper, TS: Production of cytokines by epithelial tissues. Am J Dermatopath 1989 11: 69–73,
  21. Meuer, SC, Schlossman, SF, Reinherz, E: Clonal analysis of human cytotoxic T lymphocytes: T4+ and T8-effector T cells recognize products of different major histocompatibility complex regions. Proc Natl Acad Sci USA 1982 79: 4395–4399,  | PubMed | ChemPort |
  22. Bjorkman, PJ, Samraoui, SB, Bennett, WS, Strominger, JL, Wiley, DC: Structure of the human class I histocompatibility antigen, HLA-A2. Nature 1987 329: 506–512,  | Article | PubMed | ISI | ChemPort |
  23. Yague, J, White, J, Coleclough, C, Kappier, J, Palmer, E, Marrack, P: The T cell receptor: The alpha and beta chains define idiotype, and antigen and MHC specificity. Cell 1985 42: 81–87,  | Article | PubMed | ISI | ChemPort |
  24. Gallatin, WM, Weissman, IL, Butcher, EC: A cell surface molecule involved in organ specific homing of lymphocytes. Nature 1983 304: 30–34,  | Article | PubMed | ISI | ChemPort |
  25. Jalkanen, S, Nash, GS, De los Toyos, J, MacDermott, RP, Butcher, EC: Human lamina propria lymphocytes bear homing receptors and bind selectively to mucosal lymphoid high endothelium. Eur J Immunol 1989 19: 63–68,
  26. Nakachy, M, Berg, EL, Streeter, PR, Butcher, EC: The mucosal vascular addressin is a tissue-specific endothelial cell adhesion molecule for circulating lymphocytes. Nature 1989 337: 179–181,  | Article |
  27. Streeter, PR, Rouse, BTN, Butcher, EC: Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes. J Cell Biol 1988 107: 1853–1862,  | Article | PubMed | ISI | ChemPort |
  28. Chin, YH, Rasmussen, RA, Woodruff, JJ, Easton, TG: A monoclonal anti-Hebfpp antibody with specificity for lymphocyte surface molecules mediating adhesion to Peyer's patch high endothelium of the rat. J Immunol 1986 136: 2556–2561,  | PubMed | ISI | ChemPort |
  29. Chin, YH, Falanga, V, Streilein, JW, Sackstein, R: Lymphocyte recognition of psoriatic endothelium: evidence for a tissue-specific receptor/ligand interaction. J Invest Dermatol 1989 93: 82–87,
  30. Nickoloff, BJ, Griffiths, CEM: T lymphocytes and monocytes bind to keratinocytes in frozen sections of biopsy specimens of normal skin treated with gamma infection. J Am Acad Dermatol 1989 20: 736–743,  | PubMed | ChemPort |
  31. Rothlein, R, Dustin, ML, Marlin, SD, Springer, TA: A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J Immunol 1986 137: 1270–1274,  | PubMed | ISI | ChemPort |
  32. Sanchez-Madrid, F, Nagy, JA, Robbins, E, Simon, P, Springer, TA: A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit. J Exp Med 1983 158: 1785–1803,  | Article | PubMed | ChemPort |
  33. Dustin, ML, Singer, KH, Tuck, DT, Springer, TA: Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1). J Exp Med 1988 157: 1323–1340,
  34. Stingl, G, Katz, SI, Clement, L, Green, I, Shevach, EM: Immunologic functions of Ia-bearing epidermal Langerhans cells. J Immunol 1978 121: 2005–2013,  | PubMed | ISI | ChemPort |
  35. Basham, TY, Nickoloff, BJ, Merigan, TC, Morhenn, VB: Recombinant gamma interferon induces HLA-DR expression on cultured keratinocytes. J Invest Dermatol 1984 83: 88–90,  | Article | PubMed | ISI | ChemPort |
  36. Gaspari, AA, Jenkins, MK, Katz, SI: Class II MHC-bearing keratinocytes induce antigen-specific unresponsiveness in hapten-specific TH1 Clones. J Immunol 1988 141: 2216–2220,  | PubMed | ISI | ChemPort |
  37. Dinarello, CA, Mier, JW: Current concepts in lymphokines. N Engl J Med 1987 317: 940–945,  | PubMed | ChemPort |
  38. Ritz, J: The role of natural killer cells in immune surveillance. N Engl J Med 1989 320: 1748–1749,
  39. Herberman, RB: Lymphokine-activated killer cell activity. Characteristics of effector cells and their progenitors in blood and spleen. Immunology Today 1987 8: 178–181,
  40. Kalish, RS: Nonspecifically activated human peripheral blood mononuclear cells are cytotoxic for human keratinocytes in vitro. J Immunol 1989 142: 74–80,  | PubMed | ISI | ChemPort |
  41. Saito, K, Taumura, A, Narimatsu, H, Tadakuma, T, Nagashima, M: Cloved auto-Ia-reactive T cells elicit lichen planus-like lesion in the skin of syngeneic mice. J Immunol 1986 137: 2485–2495,  | PubMed | ChemPort |
  42. Kalish, RS, Morimoto, C: Urushiol (poison ivy) triggered suppressor T-cell clone generated from peripheral blood. J Clin Invest 1988 82: 825–832,
  43. Kalish, RS, Morimoto, C: Quantitation and cloning of human urushiol specific peripheral blood T-cells: Isolation of urushiol specific suppressor T-cells. J Invest Dermatol 1989 92: 46–52,
  44. Kansenberg, ML, Res, P, Bos, JD, Schootemijer, A, Teunissen, MBM, Van Schooten, W: Nickel-specific T-lymphocyte clones derived from allergic nickel contact dermatitis lesions in man: heterogeneity based on requirement of dendritic antigen-presenting cell subsets. Eur J Immunol 1987 17: 861–865,
  45. Germain, RN: The ins and out of antigen processing and presentation. Nature 1986 322: 687–689,  | Article | PubMed | ChemPort |

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