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Journal of Investigative Dermatology (1988) 91, 181–184; doi:10.1111/1523-1747.ep12464454

Melanin Monomers Within Coated Vesicles and Premelanosomes in Melanin Synthesizing Cells

Susumu Hatta1, Yutaka Mishima1, Masamitsu Ichihashi1 and Shosuke Ito2

  1. 1Department of Dermatology, Kobe University of Medicine, Kobe
  2. 2School of Hygiene and Institute for Comprehensive Medical Science, Fujita-Gakuen Helath University, Toyoake, Aichi, Japan

Received 25 January 1988; Accepted 22 February 1988.

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Abstract

We have found substantial amounts (6.6–143 and 0.5–13 mug/mg, protein, respectively) of 5,6-dihydroxyindole (5,6- DHI) and 5,6-dihydroxyindole-2-carboxylic acid (5,6- DHI2C), which are key intermediate monomers for the formation of the eumelanin polymer, within coated vesicle fraction of pigment cells. In addition, the amounts of these eumelanin monomers have been found to decrease along with the process of eumelanin polymer formation from coated vesicles to premelanosomes and finally to melanosomes among melanogenic subcellular compartments. Our present findings seem to indicate that coated vesicles transfer not only highly glycosylated T1-tyrosinase but also eumelanin monomers into premelanosomes.

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References

  1. Mishima, Y, Imokawa, G: Role of glycosylation in initial melanogenesis: post-inhibition dynamics. In: Bagnara J, Klaus SN, Paul E, Scharl M (eds.). Pigment cell, 1985: Biological, Molecular and Clinical Aspects of Pigmentation 1985 Tokyo, Univ of Tokyo, pp 17–30,
  2. Mishima, Y, Imokawa, G: Selective aberration and pigment loss in melanosomes of malignant melanoma cells in vitro by glycosylation inhibitors: premelanosomes as glycoprotein. J Invest Dermatol 1983 81: 106–114,  | Article | PubMed | ISI | ChemPort |
  3. Ito, S, Fujita, K: Microanalysis of eumelanin and pheomelanin in hair and melanomas by chemical degradation and liquid chromatography. Anal Biochem 1985 144: 527–536,  | Article | PubMed | ISI | ChemPort |
  4. Usami, M, Takahashi, A, Kadota, K: Protein kinase and its endogenous substrates in coated vesicles. Biochim Biophys Acta 1984 798: 306–312,
  5. Mishima, Y: Micromolecular characterizations in neoplastic and dysfunctional human melanocyte. In: Della Porta G, Muhlbock O (eds.). Structure and Control of the Melanocyte 1966 New York, Springer-Verlag, pp 133–155,
  6. Hamada, T, Mishima, Y: Intracellular localization of tyrosinase inhibitor in amelanotic and melanotic malignant melanoma. Br J Dermatol 1972 86: 385–394,
  7. Ito, S, Yamamoto, Y, Fujita, K: Synthesis and antitumor activity of cysteinyl-3,4-dihydroxyphenylalanines and related compounds. J Med Chem 1981 24: 673–677,
  8. Wakamatsu, K, Ito, S: Preparation of eumelanin-related metabolites 5,6-dihydroxyindole, 5,6-dihydroxyindole-2-carboxylic acid, and their O-methyl derivatives. Anal Biochem (in press)
  9. Ito, S, Kato, T, Fujita, K, Kurahashi, T: Determination of DOPA, dopamine, and 5-S-cysteinyl-DOPA in plasma, urine, and tissue samples by high-performance liquid chromatography with electrochemical detection. J Chromatogr 1984 311: 154–159,
  10. Cunningham, P, Staehelin, A, Rubin, W, Wilkins, R, Bonneville, M: Effect of phosphotungstate negative staining of the isolated Golgi apparatus. J Cell Biol 1974 62: 491–504,
  11. Lowry, OH, Roscbrough, NJ, Farr, AL, Randall, RJ: Protein measurement with the Polin Phenol reagent. J Biol Chem 1951 193: 265–274,  | PubMed | ISI | ChemPort |
  12. Mojamdar, M, Ichihashi, M, Mishima, Y: On intracellular differentiation of melanogenic compartments for eu- and pheo-melanogenesis in melanoma cells: 5-S-cysteinyldopa formation in vitro by subcellular fractions. In: Bagnara J, Klaus SN, Paul E, Scharl M (eds.). Pigment cell, 1985: Biological, Molecular and Clinical Aspects of Pigmentation 1985 Tokyo, Univ of Tokyo, pp 717–720,
  13. Imokawa, G, Mishima, Y: Loss of melanogenic properties in tyrosinases induced by glycosylation inhibitors within malignant melanoma cells. Cancer Res 1982 42: 1994–2002,  | PubMed | ISI | ChemPort |
  14. Ohkura, T, Yamashita, K, Mishima, Y: Purification of hamster melanoma tyrosinases and structural studies of their asparagine-linked sugar chains. Arch Biochem Biophys 1984 235: 63–77,
  15. Palumbo, A, d'Ischia, M, Misuraca, G, Prota, G: Effect of Metal ions on the rearrangement of dopachrome. Biochim Biophys Acta 1987 925: 203–209,
  16. Ito, S: (unpublished)
  17. Pawelek, J, Korner, A, Bergstorm, A, Bologna, J: New regulators of melanin biosynthesis and the autodestruction of melanoma cells. Nature 1980 286: 617–619,  | Article | PubMed | ISI | ChemPort |
  18. Korner, A, Pawelek, J: Mammalian tyrosinase catalyzed three reactions in the biosynthesis of melanin. Science 1982 217: 1163–1165,  | PubMed | ISI | ChemPort |
  19. Hearing, V, Korner, A, Pawelek, J: New regulators of melanogenesis are associated with purified tyrosinase isozymes. J Invest Dermatol 1982 79: 16–18,
  20. Barber, J, Townsend, D, Olds, D, King, R: Dopachrome oxidoreductase: a new enzyme in the pigment pathway. J Invest Dermatol 1984 83: 145–149,  | Article | PubMed | ISI | ChemPort |
  21. Westerhof, W, Pavel, S, Kammeyer, A, Beusenberg, F, Cormane, R: Melanin-related metabolites as markers of the skin pigmentary system. J Invest Dermatol 1987 89: 78–81,

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